DreALTA0016884-2/2 @ danRer10
Alternative 3'ss
Gene
ENSDARG00000045803 | mfge8b
Description
milk fat globule-EGF factor 8 protein b [Source:ZFIN;Acc:ZDB-GENE-070209-137]
Coordinates
chr25:19082146-19083544:+
Coord C1 exon
chr25:19082146-19082290
Coord A exon
chr25:19083342-19083359
Coord C2 exon
chr25:19083360-19083544
Length
18 bp
Sequences
Splice sites
5' ss Seq
ACGGTAAAA
5' ss Score
6.53
3' ss Seq
TCCGCTGTCTCACTTCACAGTGT
3' ss Score
10.18
Exon sequences
Seq C1 exon
ATTTTTGTTGGTAACATGGACAATGATGGGACAAAGACGAATTTGTTCGAGCCTCCAATCATCGCTCAGTTCCTCCGGATTGTTCCTGTGGTGTGCCGTAAAGCCTGTACTCTGAGAATGGAGCTGGTCGGCTGTGAACTCAACG
Seq A exon
TGTATTTGAACACGACAG
Seq C2 exon
GTTGCTCCGAGCCGCTCGGCGTCAAGTCTCGGCTCATCAGTGACCGGCAGATGACATCGTCCAGCGCTTTCCGCACCTGGGGCATTGAGGCCTTCACCTGGCACCCGCATTACGCCCGTCTGGACAAACAGGGCAAGACCAACGCCTGGACGGCAGCTACAAACAACCGCTCCGAGTGGCTGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000045803-7-8,7-7-2/2
Average complexity
Alt3
Mappability confidence:
NA
Protein Impact
Protein isoform when splice site is used (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.061 A=NA C2=0.016
Domain overlap (PFAM):
C1:
PF0075420=F5_F8_type_C=PD(29.5=83.7)
A:
NA
C2:
PF0075420=F5_F8_type_C=PU(32.1=72.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCCGGATTGTTCCTGTGGTG
R:
CTGATGAGCCGAGACTTGACG
Band lengths:
113-131
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]