DreALTD0012264-2/3 @ danRer10
Alternative 5'ss
Gene
ENSDARG00000074622 | myo5ab
Description
myosin VAb [Source:ZFIN;Acc:ZDB-GENE-050411-72]
Coordinates
chr25:7440471-7441117:-
Coord C1 exon
chr25:7440835-7441117
Coord A exon
chr25:7440750-7440834
Coord C2 exon
chr25:7440471-7440655
Length
85 bp
Sequences
Splice sites
5' ss Seq
CATGTGAAT
5' ss Score
-0.39
3' ss Seq
TCCTTCTTGTTTTGGTGCAGGTA
3' ss Score
10.35
Exon sequences
Seq C1 exon
CACCAGCCATGCTGGAGCAGGAGACCATTCAGGGGGTGATGGGTGTAAAGCCTACGGGAATGAGGAAACGCACGTCCAGCTTCCACGAGGAAAACTCTCACTCGCTGGAGTCCATTCTGAAGCAGCTGGATGGGTTTTATTTCACTCTGCTCCAGCACGGGAATGACGCTGAGGTGGTCCGGCAGGTCATCAAACAGCAGTTCTACGTCATCTGCTCCGTCACACTCAACAACCTGCTGCTCCGCAAGGACATGTGCTCCTGGAGTAAAGGCCTGCAGATCAG
Seq A exon
GTACTGACCACTAGAGGGCACTAAAGCCTCTTTTACACTTACACACTGCTGGAAAATTACCAGTAAATTGCCACGAAGAGATCAT
Seq C2 exon
GTATAATGTTTGTCAGCTGGAGGAGTGGCTGCTGGATAAAGATCTTCAGGGCAGTGGAGCTCGAGAGTCTCTGGAGCCTCTTATTCAGGCGGCGCAGCTCCTGCAGATCAAGAAGAAGAGCCAGGACGACGCTGACGCCATCTGTACTATGTGCACAGCGCTCACCACTCAACAGGTCTGTGTGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000074622-41-41,42-41,43-41-2/3
Average complexity
Alt5
Mappability confidence:
NA
Protein Impact
ORF disruption when splice site is used (sequence inclusion)
No structure available
Features
Disorder rate (Iupred):
C1=0.063 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0184314=DIL=PU(31.1=34.7)
A:
NA
C2:
PF0184314=DIL=FE(58.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCAGGTCATCAAACAGCAGT
R:
CTCCAGAGACTCTCGAGCTCC
Band lengths:
179-264
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]