DreINT0100871 @ danRer10
Intron Retention
Gene
ENSDARG00000074622 | myo5ab
Description
myosin VAb [Source:ZFIN;Acc:ZDB-GENE-050411-72]
Coordinates
chr25:7440471-7441117:-
Coord C1 exon
chr25:7440835-7441117
Coord A exon
chr25:7440656-7440834
Coord C2 exon
chr25:7440471-7440655
Length
179 bp
Sequences
Splice sites
5' ss Seq
CAGGTACTG
5' ss Score
9.04
3' ss Seq
TCCTTCTTGTTTTGGTGCAGGTA
3' ss Score
10.35
Exon sequences
Seq C1 exon
CACCAGCCATGCTGGAGCAGGAGACCATTCAGGGGGTGATGGGTGTAAAGCCTACGGGAATGAGGAAACGCACGTCCAGCTTCCACGAGGAAAACTCTCACTCGCTGGAGTCCATTCTGAAGCAGCTGGATGGGTTTTATTTCACTCTGCTCCAGCACGGGAATGACGCTGAGGTGGTCCGGCAGGTCATCAAACAGCAGTTCTACGTCATCTGCTCCGTCACACTCAACAACCTGCTGCTCCGCAAGGACATGTGCTCCTGGAGTAAAGGCCTGCAGATCAG
Seq A exon
GTACTGACCACTAGAGGGCACTAAAGCCTCTTTTACACTTACACACTGCTGGAAAATTACCAGTAAATTGCCACGAAGAGATCATGTGAATATGGTTATGATAACAGTCCCTTTACGCATTTCCAGGTCCAAAAGGATCCATTGTAGTTGACTGATTTCTCCTTCTTGTTTTGGTGCAG
Seq C2 exon
GTATAATGTTTGTCAGCTGGAGGAGTGGCTGCTGGATAAAGATCTTCAGGGCAGTGGAGCTCGAGAGTCTCTGGAGCCTCTTATTCAGGCGGCGCAGCTCCTGCAGATCAAGAAGAAGAGCCAGGACGACGCTGACGCCATCTGTACTATGTGCACAGCGCTCACCACTCAACAGGTCTGTGTGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000074622:ENSDART00000089034:41
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.063 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0184314=DIL=PU(31.1=34.7)
A:
NA
C2:
PF0184314=DIL=FE(58.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGAAGCAGCTGGATGGGTTT
R:
CTCCAGAGACTCTCGAGCTCC
Band lengths:
243-422
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]