DreEX0013128 @ danRer10
Exon Skipping
Gene
ENSDARG00000061736 | ank3a
Description
ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]
Coordinates
chr17:20697934-20707080:-
Coord C1 exon
chr17:20707002-20707080
Coord A exon
chr17:20699999-20705869
Coord C2 exon
chr17:20697934-20698008
Length
5871 bp
Sequences
Splice sites
3' ss Seq
TATTAATTTAATTATGATAGAAA
3' ss Score
2.82
5' ss Seq
TTGGTGAGC
5' ss Score
7.43
Exon sequences
Seq C1 exon
AATGACAAAGATCGACGACATACCTTTGCCTCATTAGCATTGCGAAAGCGCTACAGCTATCTGGCCGATCCAGCATCCA
Seq A exon
AAACAGCAGAAAGAGGCCATAACACCCCTCAAAAAACCACGACGCAGCCTTCTGGCTACGCTTACAAGCCTGTCTTTACGACGCGCTCCTACCAGGCCTGGGCGACAAGCTCTCCAGTCAACGTCCCAATCCAGACCAAGTCTGGCTTTGGCTCCCTCTCCAGCTCCTCCTCCAACACCCCCTCAGCCTCCCCACTGAGGTCGGTGTGGTCTGTGAACTCTGCCTCTCCCATTAAATCTATCATTGGAGGTTCACCAGCGTCTTCCATCAAATCTGCCAGTGACGTTGCATCCCCAATTCGATCCTACAGAACAATCTCCTCACCAATAAAGACTGTAGTTCAAGGTGCTCAGTATCCAGTTCAGGCCTCCCCCACCCTTTTGTCCTCTCCAGGGAAGAGTGCCCCTGAACCAGTTTCACATAAAGGCCTCACAAGCCTGTCAGCCAGGACCTCTCCAGTGCCATCTGTTGGGACCTTGCTGGAAAGATCCTCTATAGCTATGACCCCTCCAGCCTCACCCAAGTCTTCACTAAACATGTATAGCTCAAGTTTACCATATAAGTCTGTTGTGACCTCAAACTCTCCAGCTACCTGCTCCCCAATTAAAACTGTCCCAGGCTTCTCCTCTGTCAGAACCAGCACTGATGCCTCAGTCAGAGGATACCTGTCATCCCTCTCCTCACCTCTGAAATCTGGCTCAGCCAATACAGCAGCTGCACTTATCAATGGGACAGTTTCCCCAAACAAATATCGCTCCTCCTCCCCAACAACCCATCTTACCAGCACCTTGCAGGAAAGAATTCAAGCCACAACAAATGCAGCAACAACAAGTGTGAATGCTGCCTTTGAGGAAGTGGAAAAAACATTTAATTCCTGCTCTGCTGGCTACGGCACACTAAAGTCAGTTTCCTCTACTCCCTCATCCTCTTACCAATCCATTCGTTCCTCTGCCTCCAGCTCACTCTATGCCTCCCTCAGATCACCTCCCAACTCCACAACGTCTGTAACTTCCAGCACTATTACGGTTCCTGTCTATTCAGTTATAAATGTCTTACCAGAACACCAGTTTAAAAAGCTTCCAGAAGTCTCCAAATCGACAGCCGCTCTTCTGTCTCCTAGAAAGGTCATGCCCCAAGAAGTAAATGCCCAGAATCAGTCCTCTTTTGCCAGAACTCTGTCTCCTGTCAAGACTCCCCTGCACATGTCCCCAGGAACACTAAAATCTGCTACCTCTTCATCCCCTCTGTCCAACAGTCAGGAGATACTAAAAGATGTGGCTGAAATGAAGGAGGACCTTATTAGAATGTCTGCAATTTTGCAAACTGATACAAATTCAAGCTCCAAAGGTTACCATTCTGACTCTAAGGAACATAAAGTGGAGGATGAGGAGCCTTTCAAGATTGTAGAGAAAGTAAAACAGGACCTGGTCAAAGTCAGTGAAATACTTAGCAAGGATGTTTTAAGAGAGAGTAAGGCAAATATTAAAAACCACAAAAAGGACGTCCTAGAGGATGACATTGAAGATAATCCACAAAATGAATGGAGATGCCCCCCTCGATATGAAACAGTAGCTCCTCAAGTCAAAACAAAAGCTGTATCTGACAGAGATTTTAACCTTGCAAAAGTTGTTGATTATCTAACCAATGACATTGGCACAAGTTCTCTTTCTAAAATAGCAGAGGCTAAACAAAGAACTGAGGAGGCAAGGAGAGATGGAGAGGAAAAACAGAAACGTGTCCTGAAACCTGCAATGGCATTACAGGAGCACAAACTCAAAATGCCTCCAACAAATATGCGTCCCTCACCTTCTGAAAAAGAACTTTCAAAACTAGCTGATGCATTATTTGGCCCTGAAATAGCACTGGACTCTCCTGATGATGTTTCTCATGACCAAGACAAGAGTCCGCTTTCAGATAGTGGATTTGAGACCCGCAGTGAGAGGACACCCTCAGCTCCCCAAAGTGCTGAGGGGATGGGGCCCAAAGGCCTCTTTCAGGAGATTCCCCCTGTCATCACTGAAACACGCACTGAGGTTGTACATGTCATACGTAGCTATGAACCTCCTGAAGATTCTAAGGAGCAAGCACTGGAGGATGCTAGAATGACAAAGCCTCTCTTGCGGTGTGCAGACAGTGAGCCCCGATCACCTGGTGGATCAATCAAAGAAAGAGTTAAAGCTTACCAAGCCAGAGTCAATGCAGAGGAGGATGTGATGGGCAAAGGCATGCGGGTCAAAGAGGAAACTCACATAACAACAACTACAAGAATGGTATACCACAAACCTCCCACTAAAGAGGTGGCATCTGAGAGAATGGAGGAGACCATGTCTGTACGAGATATAATGAAGGCCTTCCAGTCTGGGAAGGACCCATCCAGAGAGTTAACAGGATTGTTTGAACACAAAAGCAACAGCGAAGGAGCAGACCCATCTGCTAGGCCTCTAGACCCAATGCCCAAAGTTGAGCGCATTATTGAGGTTCACATTGAGAAAGGCAACAAAGCTGAACCAACTGAAGTGATTATCAGAGAAACAAAAAATCACCCTGAGAAGGAAATGTATATATATCAAGGCAAAAGTAGCATGAAAGAACTACAAAATGGGATGGGTGTTGGGCAACATAATTGCATAGAGCCTGAAGAATCTCTTCCTTGCTATCTAGATTCTAGAATGCACATGCCTGTCTCTGTGGAGGACGATAAGTCCTTAAAATTACTTGGTCACCAATCATTGGAATACCAAGATGATGAGTCCTCTGAAACAAGGGGAGACTCATACAAGTTTGCAGATAAAATGCTTTTGTCAGAAAAATTTGACTCTTCCCATTCAGACTCAGATGAATCTGTTATTGACCGATCTCGATACTATGGCGAGGGCACACAAGGTGGATCGATTCGAGAATTCATGCTGAAGCCTGATAGGTCACGTAGATCATCAGACGATGATTATGATAAACTCACTTTGTTGCAGTATGCTTCTGATCCTGATAGTCCAAAAAAGTCAATTTGGATGAGAGTTTCTGAGGATGGGCAGAAAAAAGGCAGAGAAAAGCAGAAATATGAGGACAGAGTAGACAGAACTGTCAAAGAGGCAGAAGAAAAACTTACTGAAGTCTCACAATTCTTTCGAGATAAAACTGAAAAACTGAATGATGAGCTTCAGTCCCCCGAGAAGAAGCATCGTAGACAAGATGCAAGGGAGACTCGATCTGGGCCCAGCTCAACTTGCAGTAGCCCAGAGAGATCAGGGCATAGAAATGGGGGGAGTGGAGAAGACTGGAGTAGAGACAGGTTTAGAGATAAGTATGGTTCTCATGATAGAAAATCCGCTAGTTTGCCTAGTAGTCCTGAAAGAAGAGTCTTGCTTCAATACAGTGATGACCCAAAAAGGGGTGACTGTTCATCTGCCAGCAGTGCCAGTGAGTCATCTAAATATTTCCAACCCTCAACATCTAAGGTTAGCTCAGTAAGAATGAAGTTTGAGTCTGAGGCCCAGAAACAAGATAAAGGGCCGCAGTGGGGACAAACTTCTGCATCTCCAGTGAGGAAGTTGCAGGAAAGTAAGCTGCCTGTTTATCAAGTTTTTGCAGGGGGGAATCTCCCTAAATCAGGAGAGTCTTCAGATAATGAAAGAAGCTCCAAAAAAGATAATGAAGGTGGGCACAGTTACAATGTTAGAAAGGCTCAAGCATTTGCCAATAATCAGCAAAGTGACAGACAAGGAAATAGGTCAGATAACTCTTCACTAAAACATCACCAAAATGTTTACAGCAAGGAGCAAATTTCTAATGAAGACAGGAAAATATACAAAACTTGGGAAAATCATGGCAATGGGCAACTGAAAGGTCAAAAGACAAAGCTCACACACACCCTAGTGCATGATAGACAAAAGGATAACTCACAGAATGAGTCTCATTTATTGAGAGAAGGTAATACCACAAAAGAAGAAACAACTAAAAAGATCATTTATACAGAGCTGGTAATCCGAGAAGACCCAAGCAATACTGAAAAATGCAATGGTGCTCTAAAAAAAAACTCGGAATCACAAATTCCTGTTAGGGTGTCATCTAGTTTTATAGATCATCATCAAAGTAGTACTTTAAAGTTAGATTCCTCCATTAAATCTGAGAGAACAGGGTCTCACAAAGCAAGAAATACACAGCACCCCAGTTCAGGTGGTACCAAATCACATAGTGATGTGTCGTCTGTAGTGAACTCAGATTCTAATAGGCAGATTGTTAATATAGTCTGTAATGGAGTGGATGAAAACCAAGTTGAGTACGTTGAAAAAGTGACCCCAGGTGCAGATATAAAACCTCTTCCTGTGTATGTCAGCATTCAAGTAGGTAAACAGTATGAGAAGGAAACCGCTACTGGGCAGCTAAGCACTTACAAAAAAGTAGTAAGCCACGAGAGCAGGACAGTACATGAAACACGTGGAGCCTTTTATTCTGTAAAACAGAAGCAGCCACAATCACCTCAGGGAAGCCCTGAAGATGACACGTTAGAGCAGGTAACATTTATGGACAGTTCAGGGAGAAGTCCTGTAACCCCTGAGACACCTACCTCTGATGAGGTCAGCTATGACCTGAACTCCAGGGCTCCTGAGCCTGTCATTGGTTCCATGGCTGGCATGCCTAGCCCAATACCAGAGGAGTCTGAGGAAGAGGAGCAGCCCAAGACATTCATTTTTAAGGAAAAACCAACAGAAAATGTCAAACCTGCCAATCATTCACCAGAATCATCAAAAAGAAAGCAAGACTCGACTGGAAAAAGGTCAAAAGACAAACGTATAGCTTACATCGAGTTTCCACCACCACCACCATTAGAGACCGAGCATGCTGACCCTGAAAAGAGGGCGTCGTGCCCTTCCTCTGAAGCAGAAACAGAGATGATAGAGGTAAACCTTCAAGAAGAACATGACAGGCATCTCCTTGCAGAACCAGTAATCAGAGTGCAGCCTCCTTCCCCTCTTCCACCTGGAGCTGATAACAGTGATTCCAGTGATGATGAATCTGTTTTTCAGCCTGCCCCACTTAAAAAGTACACCTTCAAAATGGCTGAAGAGTGTGAGAAACGTGTTAAACCTAGGAAACCAGAGAGAAACGGCTCACAACACAAGGAGTCAGGTATCAATGGCATTGTGAAAGGAGATGATGTAGACTGTGAACAAAATGGAAATGATCAATCTATCACTGATTGCTCAATAGCAACAACTGCCGAGTTTTCACATGATACAGATGCCACTGAGATAGATTCTCTTGATGGATATGATCTACAAGATGAGGACGATGGACTGAGTGACCCTAAAACATCCAGCCTGTCGAATGAGGGGAAAACAGGAGATCGTTCCTTCAACCAGAGCAAACTTGAGGTGATTGAAGAGGAGAGCACACCAAATGAGGAAACAGGAGAAAAGGCAAAAACAAAGAGGTCATCTGGTAAAAAGTCAGACAATGGCAAAGAGGATAAAATCTATTCTTTGGAGGGAAGGCACCCTGATAGACAAGGTTCGGCAGAAAATTATTTCAAGTATCAACTAGAGGAGGACATGAACACTACTTTTAAAACTGTGGCTACTAAAGGCTTGGATTTCGATCCATGGTCTGGTAAAGAGGGAGAGGAAGGAGCTTTTGATGCAAAAAATAAAGATGAAGATCCGAAGCCCTGTGCTTTATCAGTGGAAGACAAATCACAAGCTACCACACCGGATACCACTCCAGCAAGAACCCCAACAGATGAGAGCACCCCAACTAGTGAGCCCAACCCTTTCCCCTTCCACGAGGGCAAGATGTTTGAGATGACCCGCAGTGGTGCTATTGACATGAGCAAGAGGGACTTTGTTGAAGAGAGACTTCAGTTTTTCCAGATTG
Seq C2 exon
GTCCACAGAGTCCATGTGAAAGAACAGACCTACGCATGGCCATTGTGGCTGACCATCTGGGACTGAGCTGGACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000061736_MULTIEX3-3/3=2-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.432 A=0.810 C2=0.238
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0053117=Death=PU(18.1=57.7)
Main Inclusion Isoform:
ENSDART00000149630fB1180
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 1 annotated functions for this event
PMID: 25552556
Proximal axonal polarity as well as assembly of the AIS and normal morphogenesis of nodes of Ranvier all require a heretofore uncharacterized alternatively spliced giant exon of ankyrin-G (AnkG). This exon has sequence similarity to I-connectin/Titin and was acquired after the first round of whole-genome duplication by the ancestral ANK2/ANK3 gene in early vertebrates before development of myelin. Non-specific knockdown of Ank3 completely abolishes AIS clustering of AnkG, B4 spectrin, VGSC, and NF186. Rescue with 480-kDA isoform (with giant exon; MmuEX0004933) completely restores this phenotype, whereas rescue with the 270-kDa isoform (with a massive ALTD in the giant exon) partially restores the phenotype. Rescue with 190-kDa isoforms fails to restores any part of the phenotype. Specific knockdown of the 480-kDa isoform completely abolishes AnkG staining at the AIS as well as clustering of its associated binding partners, _4 spectrin, VGSC, and NF186.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]