DreEX0022163 @ danRer10
Exon Skipping
Gene
ENSDARG00000005908 | clk4b
Description
CDC-like kinase 4b [Source:ZFIN;Acc:ZDB-GENE-050227-19]
Coordinates
chr21:32251708-32256098:-
Coord C1 exon
chr21:32255941-32256098
Coord A exon
chr21:32252589-32252802
Coord C2 exon
chr21:32251708-32251774
Length
214 bp
Sequences
Splice sites
3' ss Seq
GTGGTGTTTGTTTTTTACAGGTG
3' ss Score
11.21
5' ss Seq
TCGGTAAAC
5' ss Score
4.93
Exon sequences
Seq C1 exon
ATGCGGCGATCTAAGCGGATGCGGTCACCGGATGGCTGGATTGCGGAGTACAGCTGGGAGGAGAGAGTGGAGGTGCACAAAAGACCCAAACACAGATCGCGGAGCGGGGAAAGAGAGAGGCAACCTGGCCAAACTCATCACTATAAGACATACGAGAG
Seq A exon
GTGTCATATTAGCTCAAAGAGGCTGGAGTGCAGAGAGCGCAGGGCCAGAGAGGCCAGTCTGGAGCATAGCCGTTATAAGGGTCGAGTGGACCCGGTCAGAGAGACAGAGCGGGAGAAAGATGGCTACCATTACAGCCAGTCCCGAAGTGGGCGAAGTCGGCGCAGCAGCAGAGCTCGCCAACATTCACACCACCGCAGTCCTGGCTCAGACTCG
Seq C2 exon
ATGAGATTGTAGGGACTCTGGGAGAAGGAGCCTTTGGAAAAGTCGTGGAGTGCCTTGACCACACAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000005908_MULTIEX1-1/2=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.717 A=0.958 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0006920=Pkinase=PU(6.9=95.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGCGGCGATCTAAGCGGAT
R:
TTTGTGTGGTCAAGGCACTCC
Band lengths:
225-439
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]