DreEX0022164 @ danRer10
Exon Skipping
Gene
ENSDARG00000005908 | clk4b
Description
CDC-like kinase 4b [Source:ZFIN;Acc:ZDB-GENE-050227-19]
Coordinates
chr21:32251708-32252802:-
Coord C1 exon
chr21:32252589-32252802
Coord A exon
chr21:32252066-32252156
Coord C2 exon
chr21:32251708-32251774
Length
91 bp
Sequences
Splice sites
3' ss Seq
TGATGATTCCCCAACTCCAGAGA
3' ss Score
4.49
5' ss Seq
GATGTATAG
5' ss Score
-2.49
Exon sequences
Seq C1 exon
GTGTCATATTAGCTCAAAGAGGCTGGAGTGCAGAGAGCGCAGGGCCAGAGAGGCCAGTCTGGAGCATAGCCGTTATAAGGGTCGAGTGGACCCGGTCAGAGAGACAGAGCGGGAGAAAGATGGCTACCATTACAGCCAGTCCCGAAGTGGGCGAAGTCGGCGCAGCAGCAGAGCTCGCCAACATTCACACCACCGCAGTCCTGGCTCAGACTCG
Seq A exon
AGAAGCCATCGCAGGAAAAGAACCAAGAGTATTGAGGATGATGAGGAGGGTCACCTGGTCTATCACAATGGAGACATGCTAAGAGCAAGAT
Seq C2 exon
ATGAGATTGTAGGGACTCTGGGAGAAGGAGCCTTTGGAAAAGTCGTGGAGTGCCTTGACCACACAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000005908_MULTIEX1-2/2=1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.958 A=0.516 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0006920=Pkinase=PU(0.1=0.0)
C2:
PF0006920=Pkinase=PU(6.9=95.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
CAGAGAGACAGAGCGGGAGAA
R:
GGCACTCCACGACTTTTCCAA
Band lengths:
172-263
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.725, FDR=0.000), PC9 No Change at 14 days (FC=1.106, FDR=0.829), Late Xenograft No change (FC=1.369, FDR=0.260).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]