GgaEX0008580 @ galGal4
Exon Skipping
Gene
ENSGALG00000006057 | CLK4
Description
CDC-like kinase 4 [Source:HGNC Symbol;Acc:HGNC:13659]
Coordinates
chr13:13448294-13450273:+
Coord C1 exon
chr13:13448294-13448519
Coord A exon
chr13:13449713-13449803
Coord C2 exon
chr13:13450207-13450273
Length
91 bp
Sequences
Splice sites
3' ss Seq
GATCTTGGTGCCGTTTCCAGAAG
3' ss Score
7.02
5' ss Seq
GATGTATAG
5' ss Score
-2.49
Exon sequences
Seq C1 exon
TCATTATTTGGACGATAGAACCATAAATGAAAGAGACCATCATGACCGGAGATATGTTGAGGAGTACAGAAATGACTTCTGTGAAGTATATGACCACAGGCATTATCACAGAGACTATGAAAAGAGTCACCATCATCACTATAGCAAATCCTCTGGTCGGAGCAGGAAAAGTAGTCATAAAAGGAAGCATAAGAGGCATCATTGCTCCAGTCACCAATCACATTCG
Seq A exon
AAGAGTCACCGAAGGAAAAGATCCAGGAGTGTAGAGGATGATGAGGAGGGTCACCTGATCTGTGAAAGTGGAGACGTTCTAAGAGCAAGAT
Seq C2 exon
ATGAAATTGTTGCGACTTTAGGAGAAGGCGCTTTTGGAAAAGTGGTGGAGTGCATAGATCATGATAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000006057_MULTIEX1-2/2=1-C2
Average complexity
C1*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
Show structural model
Features
Disorder rate (Iupred):
C1=0.737 A=0.484 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0006920=Pkinase=PU(0.1=0.0)
C2:
PF0006920=Pkinase=PU(7.0=95.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
TGACCACAGGCATTATCACAGA
R:
TTTCCAAAAGCGCCTTCTCCT
Band lengths:
176-267
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.725, FDR=0.000), PC9 No Change at 14 days (FC=1.106, FDR=0.829), Late Xenograft No change (FC=1.369, FDR=0.260).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]