HsaEX0015705 @ hg19
Exon Skipping
Gene
ENSG00000113240 | CLK4
Description
CDC-like kinase 4 [Source:HGNC Symbol;Acc:13659]
Coordinates
chr5:178043883-178045779:-
Coord C1 exon
chr5:178045557-178045779
Coord A exon
chr5:178044345-178044435
Coord C2 exon
chr5:178043883-178043949
Length
91 bp
Sequences
Splice sites
3' ss Seq
CTGCCCACTTGCCGTTCCAGAAG
3' ss Score
7.46
5' ss Seq
GATGTATAG
5' ss Score
-2.49
Exon sequences
Seq C1 exon
TCATTATTTAGAAGCAAGGTCCTTGAATGAGCGAGATTATCGGGACCGGAGATACGTTGACGAATACAGGAATGACTACTGTGAAGGATATGTTCCTAGACATTATCACAGAGACATTGAAAGCGGGTATCGAATCCACTGCAGTAAATCTTCAGTCCGCAGCAGGAGAAGCAGTCCTAAAAGGAAGCGCAATAGACACTGTTCAAGTCATCAGTCACGTTCG
Seq A exon
AAGAGCCACCGAAGGAAAAGATCCAGGAGTATAGAGGATGATGAGGAGGGTCACCTGATCTGTCAAAGTGGAGACGTTCTAAGAGCAAGAT
Seq C2 exon
ATGAAATCGTGGACACTTTGGGTGAAGGAGCCTTTGGCAAAGTTGTAGAGTGCATTGATCATGGCAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSG00000113240_CASSETTE1
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
Show structural model
Features
Disorder rate (Iupred):
C1=0.329 A=0.323 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0006920=Pkinase=PU(0.1=0.0)
C2:
PF0006920=Pkinase=PU(6.9=95.7)
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
CGGGTATCGAATCCACTGCAG
R:
ATGCCATGATCAATGCACTCT
Band lengths:
167-258
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
This event
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.725, FDR=0.000), PC9 No Change at 14 days (FC=1.106, FDR=0.829), Late Xenograft No change (FC=1.369, FDR=0.260).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- The Cancer Genome Atlas (TCGA)
- Genotype-Tissue Expression Project (GTEx)
- Autistic and control brains
- Pre-implantation embryo development
Other AS DBs:
FasterDB (Includes CLIP-seq data)
AS-ALPS (AS-induced ALteration of Protein Structure, links to PINs)
APPRIS (Selection of principal isoform)
DEU primates (Only for human)