DreEX0022158 @ danRer10
Exon Skipping
Gene
ENSDARG00000089372 | clk4a
Description
CDC-like kinase 4a [Source:ZFIN;Acc:ZDB-GENE-030131-576]
Coordinates
chr14:6632812-6634117:-
Coord C1 exon
chr14:6633853-6634117
Coord A exon
chr14:6633185-6633275
Coord C2 exon
chr14:6632812-6632878
Length
91 bp
Sequences
Splice sites
3' ss Seq
GCGGTGTTCCGAAACCTCAGAGG
3' ss Score
2.5
5' ss Seq
GATGTATAG
5' ss Score
-2.49
Exon sequences
Seq C1 exon
GCATTGCCTTGAGAAAAGAAGCTCAAACGAAAGACTGGACACTAGTGAAAAACACGCAGTGTTCAGAGACCATGAACACAACGGCTACGCTGCCTGTAAAGACAAGGAGAGAAACCGGGACCGATCCAGATCCCGTGAGAGACACCGGGAACGAGAGCGAGATCGAGACTGGCACCATTACAGTAAATCCTCTGGGAACAGCATGCGGAGCAGCCGGCACAGACACCGGCACAGGCACCGCCGTTCACACCCTCACTCTGACTCG
Seq A exon
AGGAGCCATCGCAGGAAAAGATCCAGGAGTGTTGAGGATGATGAGGAGGGGCACCTGGTCTATCACAGTGGAGACATGCTGAGAGCAAGAT
Seq C2 exon
ATGAGATTGTGTGTACACTTGGAGAAGGAGCCTTTGGCAAAGTGGTGGAGTGCATTGATCATGAAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000089372_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=0.516 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0006920=Pkinase=PU(0.1=0.0)
C2:
PF0006920=Pkinase=PU(6.9=95.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
TCCAGATCCCGTGAGAGACAC
R:
GCCAAAGGCTCCTTCTCCAAG
Band lengths:
179-270
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.725, FDR=0.000), PC9 No Change at 14 days (FC=1.106, FDR=0.829), Late Xenograft No change (FC=1.369, FDR=0.260).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]