DreEX0044259 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35460545-35465362:-
Coord C1 exon
chr19:35465245-35465362
Coord A exon
chr19:35463324-35463356
Coord C2 exon
chr19:35460545-35460756
Length
33 bp
Sequences
Splice sites
3' ss Seq
TTGTCTCTTGTGTCTCTCAGTGA
3' ss Score
9.11
5' ss Seq
GAGGTACCT
5' ss Score
7.63
Exon sequences
Seq C1 exon
TTCGGAGACTCACAGCAGCTTCGTCTCGTACGAATTCTGCGAAGTACAGTCATGGTTAGGGTTGGAGGAGGCTGGATGGCGCTGGATGAGTTCCTGGTGAAGAACGACCCTTGTCGAG
Seq A exon
TGACGGTCACTCCTGGCTATTTCTGCTGCAGAG
Seq C2 exon
CTCGCGGACGCACCAACCTGGAGCTTCGAGAGAAGTTCATTCTTCCAGAGGGAGCCACGCAGGGTTTGGCGGCCTTCCGGTCCCGGGGTCGCCGGTCCAAGCCTTCGTCCCGCACAGCTTCACCCACGCGCTCGTCCTCATCTGCGTCACACAGTGCCCACAGCTGCGCCTCTGTGCCCTCTGCACCCGCTACACCCACAGCCTCCTCAAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533_MULTIEX1-2/2=C1-C2
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.001 A=0.000 C2=0.757
Domain overlap (PFAM):
C1:
PF0218712=GAS2=PU(87.5=96.6)
A:
PF0218712=GAS2=PD(2.5=16.7)
C2:
PF0218712=GAS2=PD(2.5=2.8)
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGGTTAGGGTTGGAGGAGGC
R:
TGGCTCCCTCTGGAAGAATGA
Band lengths:
124-157
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]