DreEX0044258 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35460545-35465362:-
Coord C1 exon
chr19:35465245-35465362
Coord A exon
chr19:35464787-35464849
Coord C2 exon
chr19:35460545-35460756
Length
63 bp
Sequences
Splice sites
3' ss Seq
CTCTCTTTCTCTCTCCCCAGTGC
3' ss Score
13.06
5' ss Seq
TAGGTTGGG
5' ss Score
1
Exon sequences
Seq C1 exon
TTCGGAGACTCACAGCAGCTTCGTCTCGTACGAATTCTGCGAAGTACAGTCATGGTTAGGGTTGGAGGAGGCTGGATGGCGCTGGATGAGTTCCTGGTGAAGAACGACCCTTGTCGAG
Seq A exon
TGCAACATCCAGGACTTAAAATCCTGCGTTCTGACTCCAGCAGCTCCATATCATCTCGCATAG
Seq C2 exon
CTCGCGGACGCACCAACCTGGAGCTTCGAGAGAAGTTCATTCTTCCAGAGGGAGCCACGCAGGGTTTGGCGGCCTTCCGGTCCCGGGGTCGCCGGTCCAAGCCTTCGTCCCGCACAGCTTCACCCACGCGCTCGTCCTCATCTGCGTCACACAGTGCCCACAGCTGCGCCTCTGTGCCCTCTGCACCCGCTACACCCACAGCCTCCTCAAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533_MULTIEX1-1/2=C1-C2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.001 A=0.364 C2=0.757
Domain overlap (PFAM):
C1:
PF0218712=GAS2=PU(87.5=96.6)
A:
PF0218712=GAS2=PD(2.5=9.1)
C2:
PF0218712=GAS2=PD(2.5=2.8)
Main Inclusion Isoform:
ENSDART00000146394fB3676
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACTCACAGCAGCTTCGTCTC
R:
TCTCTCGAAGCTCCAGGTTGG
Band lengths:
145-208
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]