MmuEX0027449 @ mm10
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123355056-123361027:-
Coord C1 exon
chr4:123360910-123361027
Coord A exon
chr4:123360115-123360183
Coord C2 exon
chr4:123355056-123355267
Length
69 bp
Sequences
Splice sites
3' ss Seq
TTACATCTTTTTTATTCCAGTTC
3' ss Score
8.31
5' ss Seq
TAGGTTGGC
5' ss Score
2.04
Exon sequences
Seq C1 exon
TTTGGAGATTCTCAGCAGTTGCGGCTGGTCCGTATTCTGCGCAGCACTGTGATGGTCCGAGTTGGTGGAGGATGGATGGCCCTGGACGAGTTTCTAGTGAAAAATGACCCCTGCCGAG
Seq A exon
TTCATCATCCTGGGAGTAAAATAAAGCGCTCTGATTCCAGCTCTTCAGTTTCCAGTCAGTCTCCCATAG
Seq C2 exon
CACGAGGCAGAACTAACATAGAACTTAGAGAGAAATTCATCCTGCCAGAAGGGGCATCTCAGGGGATGACTCCATTCCGGTCACGGGGTCGAAGATCCAAACCATCTTCCCGGGCAGCTTCTCCCACCCGCTCCAGCTCAAGTGCTAGTCAGAGTAACCACAGCTGTACATCCATGCCTTCTTCTCCAGCCACCCCAGCCAGCGGGACCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649_MULTIEX1-1/2=C1-C2
Average complexity
ME(1-2[93=100])
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.002 A=0.917 C2=0.841
Domain overlap (PFAM):
C1:
PF0218712=GAS2=FE(49.4=100)
A:
PF0218712=GAS2=PD(2.5=8.3)
C2:
PF0218712=GAS2=PD(2.5=2.8)
Main Inclusion Isoform:
ENSMUST00000084301fB2326
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGTGATGGTCCGAGTTGGTG
R:
GTTTGGATCTTCGACCCCGTG
Band lengths:
174-243
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types