MmuEX0027449 @ mm9
Exon Skipping
Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123032299-123038270:-
Coord C1 exon
chr4:123038153-123038270
Coord A exon
chr4:123037358-123037426
Coord C2 exon
chr4:123032299-123032510
Length
69 bp
Sequences
Splice sites
3' ss Seq
TTACATCTTTTTTATTCCAGTTC
3' ss Score
8.31
5' ss Seq
TAGGTTGGC
5' ss Score
2.04
Exon sequences
Seq C1 exon
TTTGGAGATTCTCAGCAGTTGCGGCTGGTCCGTATTCTGCGCAGCACTGTGATGGTCCGAGTTGGTGGAGGATGGATGGCCCTGGACGAGTTTCTAGTGAAAAATGACCCCTGCCGAG
Seq A exon
TTCATCATCCTGGGAGTAAAATAAAGCGCTCTGATTCCAGCTCTTCAGTTTCCAGTCAGTCTCCCATAG
Seq C2 exon
CACGAGGCAGAACTAACATAGAACTTAGAGAGAAATTCATCCTGCCAGAAGGGGCATCTCAGGGGATGACTCCATTCCGGTCACGGGGTCGAAGATCCAAACCATCTTCCCGGGCAGCTTCTCCCACCCGCTCCAGCTCAAGTGCTAGTCAGAGTAACCACAGCTGTACATCCATGCCTTCTTCTCCAGCCACCCCAGCCAGCGGGACCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-'121-123,'121-121,123-123
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.002 A=0.917 C2=0.834
Domain overlap (PFAM):
C1:
PF0218712=GAS2=FE(53.2=100)
A:
PF0218712=GAS2=PD(2.5=8.3)
C2:
PF0218712=GAS2=PD(2.5=2.8)
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGTGATGGTCCGAGTTGGTG
R:
GTTTGGATCTTCGACCCCGTG
Band lengths:
174-243
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: