DreEX0045923 @ danRer10
Exon Skipping
Gene
ENSDARG00000079199 | megf6a
Description
multiple EGF-like-domains 6a [Source:ZFIN;Acc:ZDB-GENE-090312-12]
Coordinates
chr23:21215051-21217943:-
Coord C1 exon
chr23:21217815-21217943
Coord A exon
chr23:21215489-21215617
Coord C2 exon
chr23:21215051-21215179
Length
129 bp
Sequences
Splice sites
3' ss Seq
TATATATTCATCTCAAACAGCTT
3' ss Score
6.3
5' ss Seq
CCAGTGAGT
5' ss Score
8.28
Exon sequences
Seq C1 exon
AGTGTCCTCAGGGTTTCTTCGGCCAGAGCTGCCAGCACAGATGCCAGTGTGAGAATGGCGCAGTGTGTGAGCATGTGAGCGGTGCCTGCACTTGTTTGGCTGGCTGGACTGGGACGTATTGTGAAAAAC
Seq A exon
CTTGTCCTGAGGGCTTTCATGGCTTGGAGTGCCAGCAGAAGTGCCAGTGCTTGAATGGTGGCCGTTGTCATCCCGTGACTGGAGGCTGCCAGTGTCCATCTGGTTGGGTCGGGCCAGTTTGCAATGCCA
Seq C2 exon
CGTGTGAAGCTGGCACCTTTGGGCCGGCCTGCAGTCAAACCTGTAGATGCCACAATAATGGCACCTGTGACCCTGCTGATGGGCGATGTGAGTGTGGAGCAGGATGGACAGGCAGCAGCTGTGAACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079199-'24-20,'24-19,25-20
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(33.3=36.4),PF126612=hEGF=WD(100=29.5)
A:
NO
C2:
PF079748=EGF_2=WD(100=70.5)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGTCCTCAGGGTTTCTTCGG
R:
CTTGTTCACAGCTGCTGCCT
Band lengths:
257-386
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]