DreEX0050027 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23030049-23031396:-
Coord C1 exon
chr9:23031292-23031396
Coord A exon
chr9:23031088-23031192
Coord C2 exon
chr9:23030049-23030147
Length
105 bp
Sequences
Splice sites
3' ss Seq
TTGTTCATTTTCCTCTTCAGCTG
3' ss Score
10.18
5' ss Seq
GATGTAGGT
5' ss Score
4.9
Exon sequences
Seq C1 exon
CGACTTTATCGTGAAAAGTATGAGAAAGAAAAGGACAAGATCCACTCTGAGTATGACACACCTGAAATCAGACAGGTTAAGGCCACTCAACATGCAATCAGTGAT
Seq A exon
CTGTGCTATAAGGAGAAGTACTACAACACCAGAGGAACTCTTCTGCCCATGCCCATCACCCCAGAGCTGATGCACTGCTTCCACGTCAATGAGATCAACAGTGAT
Seq C2 exon
CTGAAATATAAAGAGGATCTGCATTGGCTGAGGGGCATTGGCTGCTTCATGGTTGACTCTCCTGAGATGGTTCGCATCCGTGAGATCACCAAGTTCAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630_MULTIEX3-12/26=11-13
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.286 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(39.3=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
C2:
PF0088013=Nebulin=PD(37.9=33.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGACTTTATCGTGAAAAGTATGAGA
R:
GTGATCTCACGGATGCGAACC
Band lengths:
194-299
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]