DreEX0050034 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23024667-23028772:-
Coord C1 exon
chr9:23028668-23028772
Coord A exon
chr9:23028473-23028577
Coord C2 exon
chr9:23024667-23024771
Length
105 bp
Sequences
Splice sites
3' ss Seq
CTCTCTTCTTTCAAATACAGGTC
3' ss Score
10.38
5' ss Seq
GACGTGAGT
5' ss Score
9.22
Exon sequences
Seq C1 exon
CTGCGCTACAAGGAAACATTCGAGCTGGCTAAGGGACACTCTCACGCTGATAAGGATGCCCTTGATATTCTCTGTGCCAAGAGGGTCAGAGATGACATCAGTGAG
Seq A exon
GTCAAATACAGGGAGAAGTACATCAGCACACTGGGCACCTGGAAGTCCATTCCTGACCGCCCTGAGTTCTTCCACAGCAGGATTGTGAGGGACTGCATCAGTGAC
Seq C2 exon
CTGAAATACAAGGAAGTGTATGAAAGAAACAAAGCTCAGATCAACATGGCACCTGATGCTCATGAAATCCGTGCCGCTAAAGAGGCCTACAAGAACATTAGCAAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630_MULTIEX3-19/26=18-26
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.057
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
C2:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCGCTACAAGGAAACATTCG
R:
ATTGCTAATGTTCTTGTAGGCCTCT
Band lengths:
210-315
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]