DreEX0064539 @ danRer10
Exon Skipping
Gene
ENSDARG00000005775 | scn8aa
Description
sodium channel, voltage gated, type VIII, alpha subunit a [Source:ZFIN;Acc:ZDB-GENE-000828-1]
Coordinates
chr23:27336143-27347727:-
Coord C1 exon
chr23:27347554-27347727
Coord A exon
chr23:27339672-27339785
Coord C2 exon
chr23:27336143-27336265
Length
114 bp
Sequences
Splice sites
3' ss Seq
TCATTGACCTTGCCTTGCAGAGC
3' ss Score
9.69
5' ss Seq
AAGGTGATA
5' ss Score
4.18
Exon sequences
Seq C1 exon
GCCTTTGAGGATGTGTATATTGAACAGAGGAAGACTATTCGAATCATTCTGGAGTATGCTGACATGGTCTTTACATACATCTTCATATTGGAGATGTTGTTAAAGTGGGTGGCCTATGGTTTTGTCAAGTACTTCACCAATGCTTGGTGCTGGCTGGACTTCTTCATTGTCGAC
Seq A exon
AGCGAGTGTGCTGGCAGATGAAGAGAGCTTACACTGAGGGGCCATCTCAGACGGCTCAGGGGGCCCCGGGGCCCTCCATAAAGGGGCGCTTGTGGGTTCCAACTGAGGCTAAAG
Seq C2 exon
GTGTCTATAGTCAGCCTTATAGCTAATGCGCTGGGCTACTCCGATCTAGGGCCCATTAAATCACTCAGGACACTAAGGGCCCTGAGACCCCTCAGAGCATTGTCACGTTTTGAAGGGATGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000005775_MULTIEX1-1/3=C1-3
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0052026=Ion_trans=PU(17.0=67.2)
A:
PF0052026=Ion_trans=PD(0.1=0.0)
C2:
PF0052026=Ion_trans=FE(17.4=100)
Main Inclusion Isoform:
ENSDART00000022042fB5416
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTGGAGTATGCTGACATGGTCT
R:
TCCCTTCAAAACGTGACAATGC
Band lengths:
245-359
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]