DreEX6031401 @ danRer10
Exon Skipping
Gene
ENSDARG00000052764 | chrnb3a
Description
cholinergic receptor, nicotinic, beta polypeptide 3a [Source:ZFIN;Acc:ZDB-GENE-040108-1]
Coordinates
chr1:21700380-21702901:-
Coord C1 exon
chr1:21702857-21702901
Coord A exon
chr1:21702656-21702765
Coord C2 exon
chr1:21700380-21701283
Length
110 bp
Sequences
Splice sites
3' ss Seq
GTTTAATCACTTTTTTGCAGGAG
3' ss Score
6.54
5' ss Seq
AAAGTAAGC
5' ss Score
7.31
Exon sequences
Seq C1 exon
GATGAAAAAAATCAACTGATGACCACAAATGTCTGGTTGTGGGAG
Seq A exon
GAGTGGACAGACTACAAACTGCGCTGGAATCCAGAAGACTATGGGGGAATCACATCTATCAGAGTACCCTCAGAAACGATTTGGCTGCCAGATATTGTCCTTTATGAAAA
Seq C2 exon
TGCTGATGGCCGATTTGAGGGATCTCTAATGACTAAAGCCATTGTAAGGTTTAACGGTACCATCATGTGGACTCCTCCTGCCAGCTATAAATCCGCCTGCACCATGGACGTCACCTTTTTCCCCTTTGACAGACAGAACTGCTCAATGAAGTTTGGATCATGGACATACGATGGCACTATGGTGGATCTGACCCTTGTTGACGCTTACGTGGACCGTAAAGACTTCTTTGACAACGGCGAATGGGAAATACTCAATGCCACTGGCCAAAGAGGCAGTCGACGTGACGGCATTTACTCTTATCCTTACCTCACATATTCATTCATCCTTAAGCGTCTCCCTCTGTTTTACACACTCTTTCTCATCATCCCGTGTCTCGGTTTGTCGTTTCTCACTGTGCTGGTCTTCTACCTTCCATCAGACGAAGGGGAAAAGCTTTCCCTCTCCACTTCCGTCCTTGTGTCTCTCACTGTGTTTCTTCTCGTCATTGAAGAGATCATTC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000052764-'5-3,'5-2,6-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.036
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(6.8=100)
A:
PF0293118=Neur_chan_LBD=FE(17.6=100)
C2:
PF0293118=Neur_chan_LBD=PD(55.1=37.4),PF0293211=Neur_chan_memb=PU(84.6=59.9)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAACTGATGACCACAAATGTCTGG
R:
CGTAAGCGTCAACAAGGGTCA
Band lengths:
242-352
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]