DreEX6038495 @ danRer10
Exon Skipping
Gene
ENSDARG00000078475 | klhl23
Description
kelch-like family member 23 [Source:ZFIN;Acc:ZDB-GENE-081104-458]
Coordinates
chr9:48477436-48487088:-
Coord C1 exon
chr9:48486765-48487088
Coord A exon
chr9:48480629-48481846
Coord C2 exon
chr9:48477436-48477588
Length
1218 bp
Sequences
Splice sites
3' ss Seq
ATAATCCTCTCTTTTTCCAGTTG
3' ss Score
10.07
5' ss Seq
AAGGTATGA
5' ss Score
9.11
Exon sequences
Seq C1 exon
CTAAAACGTGTACACTAGCTAGTATTGTGTCTCAGTTCAGTCGGAAAGCGGTAAAATTGTGTCTTATTAATGCTGGGATGCTCGACTGGTCACATAGGCTGAAAGACGCGGCTGTGTTGGATCACTCTATAGCTGGTGACAGCAGCAAGTCTCCGGCTTTCAACTGAGACCAAAATAGGAGCTCGACATTTTGTAATCCTGCAGCCGACGCCAAATATACAGGAGCCACACATGTCTGATTTGGCCAAACACAAGGCGCTCTGTTGGGGATACAATCTATTCGGTGAGCTAAGGACAAGCTGAGTTAACTAGATAGATGGTCAG
Seq A exon
TTGCGATGTCCGGGAATGCTCAAAGCGGTTACTCCTACGACTTCTACGATGCCTCTCACCCTTCAGAAGTGCTTGAAGCTCTTCACGAGTTTCACAACAGCGGCCTGTTCACCGACATCGCCCTGCAGTCCTCATCGGGACAGCTCTTCCACTGCCACAAAGCCGTCCTATCAGCGCGCAGCTCCTATTTCAAAGTAATGTTCACCTTAGACATGAGAGAACGTGCGAACGACACCATCAACCTACCTTGCGTAGACGGAGAAATTTTGGGTGCTTTGGTGACCTACGTGTACACCGCCAAAATCGGCATAACTCAAAACAACGTACAACGTCTACTGGAGGCCGCAGACCTGCTGCAGTTCAACTCGCTCAAGAAAGCCTGCGAAAACTTTTTGATTCGTCTGCTAGATGTTGATAATTGCCTCGGGATGCATTCGTTCGCTGAGCTTCATGTTTGTTCAGCATTGGAAAGAGCGGCTTTGAGGATCATCCTGAGTCGTTTTGAAGACGTCACGCTTCAGGATGAATTCCCAGAGACCGGTTTTCAAAAGCTGATGGCGATTTTATCAACAGAGAACCTAAATGTGTGGAAAGACACAACGTTATTGGATGCGGTCGTGAAATGGGTCGTTTATGACGTAGCTTCTCGAATTGATCACATTCAAGAGCTCCTCAACTGCATTCATATTAACGTAGACGATGTTTATCTGAAAATGGCGTTAGATCTGCGCAAATGTTGCTCTGAGAGCAAACTGAGATCTTTAATTCTGACCTCCTTGAAACCAAGTAAAGGTTTTTCAGACTGTTGCAAGAAGCCGACATGCAGTTTGTATGTTATCGGTGGATATTATTGGCATCCTTTGTGCGAAGTTCACATGTGGGACCCCGTTTCCAACACGTGGGTTCAGGGAAAAGACATGCCTGATTTTGCAAGGGAGAGCTATAGTGTGGTTCTTCTAGGGGCTGATATTTATGTGACCGGAGGGTACAGGACAGAGACTGTCGATGCTTTAGATAATGTGTGGATTTATAATACAGATTCAGATGAATGGACTGAAGGATGTCCGATGATCACAGCTAGGTATTATCACTGCTCTGTGGCGTTACGTGGATGTGTATATGTGATAGGTGGATATACAGCAGGCGCTCCTACTCAAGAAACTGAATTTTATGATCCTTTGAAGAAGACTTGGTTTCCGGTGGCTGAAATGATACAAG
Seq C2 exon
GTGTGGGAAACGCAACCGCTTGTGTGGTGAATGACAGAGTATATGTAACTGGAGGGCATTATGGGTACAGAGGAACCTGCACTTATGAGAAGATTCAGACTTACAGGCCAGACATCAATGAGTGGAGCATCACAACAATCTGTCCACATCCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000078475-'3-3,'3-0,4-3=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion (Ref, Alt. ATG (<=10 exons))
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NA
A:
PF0065126=BTB=WD(100=26.9),PF0770710=BACK=WD(100=24.9),PF0134420=Kelch_1=WD(100=8.1),PF0134420=Kelch_1=WD(100=11.9),PF0134420=Kelch_1=WD(100=11.4),PF0764610=Kelch_2=PU(0.1=0.0)
C2:
PF0764610=Kelch_2=WD(100=90.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCACATAGGCTGAAAGACGCG
R:
ACTCATTGATGTCTGGCCTGT
Band lengths:
358-1576
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]