DreEX6050303 @ danRer10
Exon Skipping
Gene
ENSDARG00000011672 | sema3b
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B [Source:ZFIN;Acc:ZDB-GENE-081105-78]
Coordinates
chr8:26564692-26570029:-
Coord C1 exon
chr8:26569974-26570029
Coord A exon
chr8:26568496-26568635
Coord C2 exon
chr8:26564692-26568312
Length
140 bp
Sequences
Splice sites
3' ss Seq
TCTTCATGCGTGTCCCACAGACC
3' ss Score
10.49
5' ss Seq
GAGGTGAGA
5' ss Score
7.66
Exon sequences
Seq C1 exon
GAGGTTCCGGCGTCAGGATGTGAGGAACGGAGATCCTAATGCCCTCTGCTCAGGAG
Seq A exon
ACCATCAGAAGCAGCGTGTCCTGGAGAAGAGGTTGTACGCTGTGGATGGAAGCAGCTCCTTCCTGGAATGCGTCCCGAAATCACTGCAGGCCCAGATTACATGGACCTATCAGAAGCTTCCGGAAAACCCACGGGAGGAG
Seq C2 exon
GTGCGTCTGGATGAGCGTGTTCTGCAAACTGAGCGAGGGTTATTGCTGAGGCGAGCTGTGCGTAGAGACGCCGGTGTATATCAATGCCACTCTATGGAGCACGGATTCACCCAGACTATCCTGGCCATCACACTGGAGATTTTGCCTTCGGCTGGCTCCGCCCCTTCTGCTGTTAACCCCCGCAGCCCCGCCCACTCACATGTCAGTCATGGCCAGTCCACCAATCAAAAACTGTGGTACAGGGACTTTATGCAGTTGGTCGATCATCCAAACTTAAACACAGTCGACCAGATCTGTGAACAAGTGTGGTCGCGGAAGCACAGCCTATCAGGGAAGAGCCCACCAGATCCAGCCATCGAGGTGCCAGCTCATGATCCGCTGAACTCCAAAAAGTGGAAGCACCTAAAGGAAATGAGGAAGGGCCGAAATCGCCGAACACACGATGGCAAACCTGCATCCAGAGCACCGCGGAGCGCAGGAGAGTAGCGAAGGATAAAACT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000011672-'16-21,'16-20,17-21=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.350 A=0.000 C2=0.522
Domain overlap (PFAM):
C1:
PF0143720=PSI=FE(32.2=100)
A:
PF0143720=PSI=PD(6.8=8.5),PF0248011=Herpes_gE=PU(22.7=53.2)
C2:
PF0248011=Herpes_gE=PD(75.5=51.2)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTAATGCCCTCTGCTCAGGA
R:
TGGACTGGCCATGACTGACAT
Band lengths:
242-382
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]