BtaEX6095189 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000010138 | SEMA3B
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B [Source:HGNC Symbol;Acc:HGNC:10724]
Coordinates
chr22:50655648-50656676:-
Coord C1 exon
chr22:50656621-50656676
Coord A exon
chr22:50656389-50656528
Coord C2 exon
chr22:50655648-50656052
Length
140 bp
Sequences
Splice sites
3' ss Seq
GACATTCCCTTTGCCCCCAGACT
3' ss Score
9.32
5' ss Seq
CAGGTGAGC
5' ss Score
9.6
Exon sequences
Seq C1 exon
GCGGTTCCGACGGCAGGACGTAAGGAACGGCGACCCCAGCACACTGTGTTCCGGGG
Seq A exon
ACTCATCCCACCCTGTGCTGCTGGAGCAGAAGGTGCTGGGTGTGGAGGGAGGTAGCGCCTTCCTGGAGTGTGAGGCCCGCTCGCTGCAGGCGCACGTGGAGTGGACCTTCCAGCGCGCGGGGGAGTCGGCCTACACTCAG
Seq C2 exon
CAGGTGCCTGCGGAAGAGCGAGCTGAGCGCACGAGGAGGGGATTGCTGCTGCGCGGGCTGCGGCGTGGGGACTCCGGCTTGTACCTGTGTGCAGCGGTGGAGCAGGGCTTTTCGCAGCCACTGCGTCGCCTAGCGCTGCACGTGCTGAGTGCAGCACAGGCTGAAAGACTGGCCCGGACCGAGGATACTGCGCCAGTCGCCCCGCCGGGCCCCAAGCTCTGGTACCGGGACTTTCTGCAGCTGGTGGAGCCCGGCGGTGGTGGTGCGAACTCCCTGCGAATGTGTCGCCCTCAGCCCGCGCCGCGCCCACAGGTCCCTGAGTCACGGAGAAAGGGCCGCAACCGGAGGACACACGCCCCAGAGCTGCGGGCTGAGCGGGGCCCACGCAGCGCAGCGGACTGGTGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000010138-'25-36,'25-33,26-36=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref, Alt. Stop)
No structure available
Features
Disorder rate (Iupred):
C1=0.300 A=0.043 C2=0.530
Domain overlap (PFAM):
C1:
PF0143720=PSI=FE(29.2=100),PF0768612=V-set=PU(11.2=60.0)
A:
PF0143720=PSI=PD(15.4=21.3),PF0768612=V-set=FE(43.0=100)
C2:
PF0768612=V-set=PD(43.9=34.8)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACCCCAGCACACTGTGTTC
R:
AAGTCCCGGTACCAGAGCTTG
Band lengths:
258-398
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]