DreEX6080183 @ danRer10
Exon Skipping
Gene
ENSDARG00000079199 | megf6a
Description
multiple EGF-like-domains 6a [Source:ZFIN;Acc:ZDB-GENE-090312-12]
Coordinates
chr23:21194407-21195818:-
Coord C1 exon
chr23:21195730-21195818
Coord A exon
chr23:21195520-21195648
Coord C2 exon
chr23:21194407-21194663
Length
129 bp
Sequences
Splice sites
3' ss Seq
CTTTTTTCTGCATGCATCAGGCT
3' ss Score
4.39
5' ss Seq
AAGGTACTT
5' ss Score
8.4
Exon sequences
Seq C1 exon
ATGTGACTGTGATGGAGACACCCCATGTGACCCTATTAATGGCAGGTGTTTGTGTGCACCTGGAAAAACAGGCTCTCGCTGTGACATAG
Seq A exon
GCTGTGGAGTGAACCGATTTGGACCCGACTGCTCACAGAGATGTGAATGTCTGAACGGAGGTCAGTGTGATCCCCGAAACGGCCGATGCACGTGTCTGAACGGCTGGATCGGGCCCAGCTGTCAACAAG
Seq C2 exon
ATTATATGACAAACTCAAACACCGACCATGACAGAGAGATACTCATTCTTCCTGATGTATGAAATACTGGAAGAGAATCTAAAAGAAAATCCTGAATATGGAACCCGTTGTCTTCAGAAAAGTTGAGATTTTATGTGATTTTATCTGTGTATAATGCTTATTAAAACAATAGCGCAGTTGCTTCAGTACAGTAATCAAGTAAGCGTTGTTTTGCTTCTGTTTTATGAGCGCCCCCTGCTGGTAAAGAATAAATAAGC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079199-'38-35,'38-33,39-35=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.050
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(30.2=29.5)
A:
PF079748=EGF_2=WD(100=70.5)
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACAGGCTCTCGCTGTGACATA
R:
GGGGGCGCTCATAAAACAGAA
Band lengths:
257-386
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]