DreINT0035465 @ danRer10
Intron Retention
Gene
ENSDARG00000015174 | atp6v0a1b
Description
ATPase, H+ transporting, lysosomal V0 subunit a1b [Source:ZFIN;Acc:ZDB-GENE-050522-215]
Coordinates
chr24:7587444-7589234:-
Coord C1 exon
chr24:7589018-7589234
Coord A exon
chr24:7587552-7589017
Coord C2 exon
chr24:7587444-7587551
Length
1466 bp
Sequences
Splice sites
5' ss Seq
CAGGTAACA
5' ss Score
8.88
3' ss Seq
GTTTGTCTGTGTGCGCATAGGCT
3' ss Score
7.81
Exon sequences
Seq C1 exon
ATACTTTAAGAAGCCTCTGAACATTTTCCTGAATTTCATCCCTGAGATTGTGTTCATGAGCAGTCTTTTCGGTTATCTGATCCTGCTCATCTTCTACAAGTGGATCGCTTATGATGCGGTGACCTCTAAAGACGCTCCCAGTCTTCTGATAGCCTTCATCAACATGTGTCTGTTCAACTACAACGACCCCACCAACAAACCGCTGTACAGAGGACAG
Seq A exon
GTAACACACATTAACGCAAAACAGAAGTTGTATTAATAGATCATTCTTGTTATATGCAGTTTGTACAAATATCAGAAGTTCAGTAAATTTTTCACTCTTTGTTATATTGCAGCCTTTTGCTAAAACAATTTCAGTTTGTTTTTTTCCCTCATTAATGTACACACAGCACCCCATATTGACAGAAAAACTCAGAATTGTTGACATTTTGCAGATTTATTAAAAAAGAAAAACAGAAATGTCACATGGTTTTAAGTATTCAGACCCTTTGCTCAGTATTTAGTAGAAGCACCTTTTTGATCTAATACAGGCTTGAGTTTTTCACACCTGGATTTGGGGATCTTCTGTCATCCTCTTGCAGATCCTCTCCAGTTCTCTCAAGTTGGATGGTAAATGTTGGTGGATAGCCATTTTTAGGTCTCTCTATCGATGCTCGATTGGGTTTAAGTCAGGGCTCTGGCTGGGCCATTCAAGAGCAGTCATGGAGTCGTCGTGAAGCCATTCCTTAGTTGTTTTAGCTGTGAGCTTAGGGTCATTGTCATATCAGAAGGTAAAGCTTCGGCCCAGTCCTTAGCACTCCGGAGAAGGTTTTCATCCAGGATATCCCTGTACTTGGCCGCATTCATCTTTCCCTCGATAGCAACCAGTCATCCTGTCCCTGCAGCTAAAAACACCCCCACAGTATGATGCTGCCACCACCATGCTTCACTGTTGGGAGTGTATTGGACAGGTGATGAGCAGTGCCTGGTTTTCTCCACACATACCTCTTGGAATTAAGGTCAAAACATTCTATCTTGGTCTCATCAGACCTGATGACCTTTTAGTAGAGGCTTTATAAAGCCCAGACTGGTGGAGGACTGCAGTGATGGTTGACTTTCTATAACTTTCTCTCATCTCCCGACTGGGTCTCTAAAGCTCAGCCTCAGTGATCTTTGGGATCCTTTTTACCTCTCTCACCAAGGAGGTTCTTTCCAATTGCTTAGTTTGGCTGGACAGCCAGCTCTAAGGGTTCTAGTCGTCCCTAACGTCTTTTATTTAAGGATTATGGAGGCCACTGTGCTCTTTTTGTAACCTCGGCCAGATCGGTGCCTTGCCAAAATTCTGTCTCTGAGATCTTTAGGTAGTTCCTTTGACCTCATGATTCTTATTTGCTCTGACATGCATTGTGAGCTGTAGGGTCTTATTTAATATTATCTAATCAAGTCCAACCAGTATAGGGTCTGAATACTTAGGACCTTGTAATTTTGCAGATTTATTAATAAAGAAAAACTGAAATAAGTCAACAATTCTGTGTTTTTGGGGTGCTGTGTGTACATTAAGAGGGGGAAAATTAAATGATTAAATGAGTTTAGCAACTGGCTGCAATATAACAAAGAGCGAAAAATTTAAGGCGGTCTGAATACTTTTCGTACGCACTGTATATGACTTCTGGCTGGATGAATAAAAGATGTTTGTCTGTGTGCGCATAG
Seq C2 exon
GCTGGGATTCAGTCTCTGTTGGTGGTGATAGCTTTAGCCTGTGTGCCAGTTATGCTGGTGGTGAAGACTATGATCCTGCGCCGTCAGCACTTGTGGAAGAAGCACCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015174:ENSDART00000041714:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=FE(8.9=100)
A:
NA
C2:
PF0149614=V_ATPase_I=FE(4.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TAAGAAGCCTCTGAACATTTTCCTGA
R:
AGGTGCTTCTTCCACAAGTGC
Band lengths:
318-1784
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]