DreINT0035470 @ danRer10
Intron Retention
Gene
ENSDARG00000015174 | atp6v0a1b
Description
ATPase, H+ transporting, lysosomal V0 subunit a1b [Source:ZFIN;Acc:ZDB-GENE-050522-215]
Coordinates
chr24:7572842-7574959:-
Coord C1 exon
chr24:7574842-7574959
Coord A exon
chr24:7573014-7574841
Coord C2 exon
chr24:7572842-7573013
Length
1828 bp
Sequences
Splice sites
5' ss Seq
CTCGTAAGT
5' ss Score
9.63
3' ss Seq
GGATTTTTTGTCTCTATCAGAGT
3' ss Score
7.89
Exon sequences
Seq C1 exon
TTTAACTTTGGAGACATGGCTGTTCATCAGGCCATTCACACTATAGAGTATTGCCTGGGCTGCATCTCCAACACGGCCTCATACCTGCGACTCTGGGCCCTTAGTTTGGCTCACGCTC
Seq A exon
GTAAGTGCGCACACACACATATACACGCTTGTTTTTGTGAATTGTGAAGACATTCCATACACTAAATAAAGTAAAACTACTGTGTTGTTCATTCAGTGTAAATGTAAATTTGGTTTCCCCTGGGCAGAGTGAATGTTTTGATTAATATTAAATGACATTTTTCAGGATCAGCCAAGTCCATTTTAAAACAAATACTTCATTGATCTAAGAGTAAAAAAGTTTTTCCATATTAAAAATTTCCTTTGTGCACTCACAGGCCACTTTATTAGGTACAGCTGTCCAACTGTTCTGTTAACGCACATTTCTAATCAGCCAGCAGTTGAATGCATTTAGGCATGTAGACATGGTCAAGACGATGTGCTGCAGTTCGAAACGAGCATCAGAATGGGGAAGAAAAGTGATTTTAGTGACTTTAAATGTGGCATGGTTGTTGGTGTCAGACAGGATGGTCTGAGTATTTCAGAAACTGCTGATCTACTGGGATTTTCACGCACAACCATATCTAGGGTTTACAGAGAATGGTCCAAAAAAGAAAAAAAATATCCAGTGAGTGGCAGTTCTGTAGGTGCGAATGGCTTGTTGATGCCAGAGGTCAAAGGAGAATGGCCAGACTAGTTGAGCTGATGGAAAAGCAACAGTAACTCAAATAAGCACTCGTTACAACTGAGGTCTGCAGAAGAGCATCTCTGAACATATCCAACCTTGAGGCTACAGCAGCAGGAGACCACACTGGGTGCCACTATTGTCAGCTAAGAACAGGTCAGTGAGGCTACAATCGCACAGGTTCAACAAAATTGGACAATAGAAGACTGGAAAAATATTTCCTGGTCTGATGAGTCTCGATTTCTTCTTGACATTTGGATATTAGATTCAGAGATTGGCGTCAACAACATGAAAGCATGGATCTATCCTTGCTGTATCAACAGTTCAGGCTGCTGTTGTAATTATGTGCTGGATATTTTCTTCGCACACTGTGGGCCCATTTGTACCAATTGAGCATCGTGTCAACTCCACAGCCTACCTGAGTATTGTTGCTGACCATGTCCATCCCATATGGCCACAGTGTACGCATCTTCTGATGGCTACTTTCAGCAGGATAACACACCATGTCATAAAGCTTGAATCATCTCAGACTGGTTTATTGAACAGTACAATGAGTTCACTGAACTCAATGTTCTCTACAGTTACTAGATCTCAATCCAATAGAGCACCTTTGGGATGTGGTGAAACAGGAGATTCACATAACAAATGTGCAGCTGACAAATCTGCAGCAACTGCGTGACGCTATCATGTCAGTATGGACCAAAATCTCTGATGAATATATTCAGTAGCTTGTTGAATTTATACCACGAGCGATTAAAACAGTTCTGAAGGGAAAAGGGAGTCCAACCTGGTACTAGTGAGGTGTACCTAATAAAGTGGCCGGTGAGTGTATGTTGAATGTAAATATTTTGGATCTGAATTCAATTGTACTTACAATACTTACAAAAATTGTCGTTTTTTTAAAGTCAGTTCCAAGTGTATTTTTTTTACATTGAAGGTTTCTATTGTTTTGATACTGTACAAGCTGTATTTTCTATCACCCTGCCACTCACAGAAAACTATGTGCATCCTTACTCTCTGGTAAAAATCATTCTGTATGATTTATAAACCTTTTAAAAAACTGGGCATATGTCCGCATATATCACCTTCACCTTGTAATATCTACAGTATGCATTGCCCATGTTGCTATACTAAATTGTGTCGTTATATGTCACAAAAACACACATATATCAACAGCACATCCACTGGCTTATTGAAATGTAACTGGATTTTTTGTCTCTATCAG
Seq C2 exon
AGTTGTCTGAGGTGCTTTGGGGAATGGTAATGCGTCTTGGACTGTCCTCTAGAAGTGGTGGGGGATTCTTTGGCTTGTCTATCATTTTCTCTGCCTTTGCAACGCTAACAGTGTGCATCTTACTCATCATGGAGGGATTGTCTGCCTTCTTGCATGCTCTGCGCTTGCATTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015174:ENSDART00000041714:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=FE(4.8=100)
A:
NA
C2:
PF0149614=V_ATPase_I=FE(7.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TAACTTTGGAGACATGGCTGTTCA
R:
TGCAAGAAGGCAGACAATCCC
Band lengths:
270-2098
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]