DreINT0039095 @ danRer10
Intron Retention
Gene
ENSDARG00000021004 | c5
Description
complement component 5 [Source:ZFIN;Acc:ZDB-GENE-120510-2]
Coordinates
chr5:62780077-62782221:-
Coord C1 exon
chr5:62782009-62782221
Coord A exon
chr5:62780162-62782008
Coord C2 exon
chr5:62780077-62780161
Length
1847 bp
Sequences
Splice sites
5' ss Seq
CTGGTGAGA
5' ss Score
7.54
3' ss Seq
AACTCACTGTTGCTTTACAGGCA
3' ss Score
8.46
Exon sequences
Seq C1 exon
GTGAAGTACTGGGAGATATTCTGTCAATACTGAATAACCCAGAAGGTATAAAAATGCTGACCAATCTGCCAAGGGGAAGCGCCGAGCTGGAGCTCATGAGTCTCCTGCCAGTCTACTATGTCTATCACTACCTTGACAAGACGGAGAAATGGAACATTTTGGGGAAAGAGGCGGCCGCGAGTGAAAAGGAACTGAAGAGAAAGATGCAAGCTG
Seq A exon
GTGAGATGCTTGTTACACTATAAGGCAGAATTAAAATGATTGACCTTATCATTACATTCTGTGTCGAATAATAGGTACACTGTAAAAAAATGCATTCATTGATTTTCCTTCAGCTTAGTCCCTTATTTATTAGGGGTCGCCACAGTGGAATGAACCACCAACTATTCCATCATATGTTTTGCAACCCAGTACTGGGAAACACCCATACACAGTCATTCACACACACACACACACACACACACACACACACACACTCATACACTACAGCCAATTTAGTTTGTTCAATTCACCTATAGTGCATGTCTTTGGGCTTTGGGGGAAACCAGCGAACCTAGAGGAAATCCAAGCCAACACTGGGAAAATATGCAAACTCCAGACTGGCCCAGTCGAGAATCGAACCAGTGACCTTCTTGCTGTGAGGTGACAGTGCTAACCACTGAGCCACCGTGCCGCCCCTGTAAATTAAACATTTTTAGTTGAATCAAATTAACTTTTCTAGTCATCTTAACTTAACATTAATAAAACGGACTTAAAATATGAAGTTAAACTTTGCAGAAAAAAAGGAGATAAAAAGCAGATTACCTTGTTAAAATAAGTTAAAGCAACATAAACATAATTGTTGTTTTGGCTTTTTGTCATAATTTTATATCAGTGTAGGCCCATGTAGAATCTGCGCTCTCTGAAATCCACTAACTATTTTTGAGTTCATCACTGAGTCTATTTTTTTACTCATGTACATGTGTGTAGATACTTGTTCAGTTTTTATATAAATTTCAGTAATATTATTGAATTATATGAAATTGTTTTGGGGGCTGCACGGTGGCATAGTGGGTAGCGCTGTCGCCTCACAGCAAGAGGGTCGTTGGTTCAGGCCTCAGCTGGATCAAGCTGGGTCAAGGGCATTCGCTCCGTAAAACATATGATGGATAAGTTTGCGGTTCATTGCGCTGTGGCAACCCCATATTAATAAAGTGACTTAGCCAAAAAGAAAATGAATGGATGTTTTGATTGTACCATACAATTTGTGAAGTAATATTGTCTGTCATTTAGAGGGTGTATTATATAAGAGAGCTACAGCTTTGTTTAACAAACAAGTCATTGGTTTTGCATTGTAAACCTGAAGTAAAAGTTGAGACGTTTTTTTATTTTTAATTTTATTGGTCACCTTTTCTGCATAAATCTTACATTTTAAATAAATCAAATTTTTATTATTATTTTTTTTAAATCTTCATAACAGAAATAAAAATAAAAAAGTCTCCAGTTTCTGCATGACCCTGCTAATAGAATTTCTTTTTTCATTATTCATTTTATTTATTCATTCGTTCATTTTCTTTTCGGCTTAGTCCCTTTATTAATCAGGGGAATGAACGGCCAACTTATCCAGCATATGTTTTTTACAGCGGATGCCCTTCCAGCTGCAACCCATCACTGGGAAACATCCATACACGCTCATTCACACACATGCGTTATACACAATTTAGTTTATTCAATTCATCTATAGAGCATGTCTTTGGACTTGTCGGAGATACCGAAGCACCTGGAGGAAACCCACACCAACACGGGGAGAACATGCAAACTCCACACAGAAATGCCAACTGACCCAGCCGAGGCTCGAACCAGAGACGTTCTTGCTGTGAGGCGACAGCGCTACCCACTGCGCCACCACACCGCCATATTTATTTTATTACTATTTACAATTACATTTTTCCACAAAATAGTATAATTGTAATGATAAATCAATATCAATATTTTTCAGTTTTATAATAAAAAGCACTGTGTTTGGTTATTATCAATATTAGTGCTAATTATTTTTCCAGAAAGCAATACAACTCACTGTTGCTTTACAG
Seq C2 exon
GCATCACCAGCATCATGTCATTTCAGCTGAAGAATGGAGAAGCCTTCAGCATGTGGGCACAAAAGGACAAAACCCCCAGCACCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000021004:ENSDART00000097325:25
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF076789=A2M_comp=PU(0.4=1.4)
A:
NA
C2:
PF076789=A2M_comp=FE(10.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGAAGTACTGGGAGATATTCTGTCA
R:
GTGCTGGGGGTTTTGTCCTTT
Band lengths:
295-2142
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]