DreINT0045456 @ danRer10
Intron Retention
Gene
ENSDARG00000058992 | cers2b
Description
ceramide synthase 2b [Source:ZFIN;Acc:ZDB-GENE-110408-40]
Coordinates
chr16:1362401-1362742:+
Coord C1 exon
chr16:1362401-1362519
Coord A exon
chr16:1362520-1362684
Coord C2 exon
chr16:1362685-1362742
Length
165 bp
Sequences
Splice sites
5' ss Seq
CAGGTCTTT
5' ss Score
2.36
3' ss Seq
ACCCTTTTGTTCTTTTATAGCTG
3' ss Score
11.25
Exon sequences
Seq C1 exon
AGTTCAGTGGAGAAGCTGAGCAAGCAGACGGGATGGACAGAGCGGCAGGTCCAGCGCTGGTTCAGACGCCGCAGAAATCAAGACAGACCAAACCTGCTGAAGAAGTTCTGCGAGGCCAG
Seq A exon
GTCTTTTCTCTAGCTTTGAACTAAGAGAGTGTGTCTGAGCCTCGGACATTATCAGGAAGCCTATTCTTGAGTTTAGGAGCCATATATGAGAAGGCTTTACCTCCTTTAGTGGATTTTGCTGGACTTTGTACATCAACCCTAACTGACCCTTTTGTTCTTTTATAG
Seq C2 exon
CTGGAGATTCGCCTTTTATCTTCTGGCCTTCATCGGTGGTCTTGCTGCATTAATAGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000058992:ENSDART00000149299:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.075 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0004624=Homeobox=PD(58.0=72.5),PF0379811=TRAM_LAG1_CLN8=PU(2.6=12.5)
A:
NA
C2:
PF0379811=TRAM_LAG1_CLN8=FE(9.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTCAGTGGAGAAGCTGAGCA
R:
TTAATGCAGCAAGACCACCGA
Band lengths:
171-336
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]