DreINT0045460 @ danRer10
Intron Retention
Gene
ENSDARG00000058992 | cers2b
Description
ceramide synthase 2b [Source:ZFIN;Acc:ZDB-GENE-110408-40]
Coordinates
chr16:1367472-1368138:+
Coord C1 exon
chr16:1367472-1367600
Coord A exon
chr16:1367601-1368031
Coord C2 exon
chr16:1368032-1368138
Length
431 bp
Sequences
Splice sites
5' ss Seq
GAGGTCAGA
5' ss Score
5.24
3' ss Seq
TCTCTCTCTCTATCTCTCAGTCA
3' ss Score
9.47
Exon sequences
Seq C1 exon
GACTTTAAAGAGCAGATCATCCATCATGTTGCCACCATCCTGCTCATCAGCTTCTCCTGGTGTGTGAACTACATCCGAGCAGGAACGCTCATCATGTTCATGCACGACTCAGCCGACTATCTGCTGGAG
Seq A exon
GTCAGAACAACACACACACACACACACACATCTCACAACACACACTACACTGTACACATGCACACAGATCTGCTGAATGAAGACTGAGGTGCGATTAAGGGAAGGCAACGACTGACTGTGAACATGAAGAGAATAAAGACAACACTTTTAGACCATGCGCTGGCTGTGCTAGCTGCTTTACCATCCCGAAACAAGCCAAAGTGGAACTGCGCTGAGTGCAACATTCATTTAATTTCCAATATTTAAAATGTTGTTATGTGGAAGACAACACAAATGCACACTTCAGAAATCCACAAGAATTAATAATCAACCCAGTACTCGAATATTCCACAGCTTGATCTGTTTCACCAGAAAAACTATTCCCGTCTCTCTCTCATATATCTGAACACATCAGTTTCTCTCCTCATGTCATCTCTCTCTCTATCTCTCAG
Seq C2 exon
TCAGCTAAGATGTTTAACTACGCCCGATGGAAGAACGCATGCAACTACATCTTCATCCTGTTTGCTGCCATCTTCATCGTCACACGCCTCATCATCTTCCCTTTCCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000058992:ENSDART00000149299:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0379811=TRAM_LAG1_CLN8=FE(21.6=100)
A:
NA
C2:
PF0379811=TRAM_LAG1_CLN8=FE(18.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAAGAGCAGATCATCCATCATGT
R:
CGGAAAGGGAAGATGATGAGGC
Band lengths:
230-661
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]