DreINT0045492 @ danRer10
Intron Retention
Gene
ENSDARG00000002365 | cers5
Description
ceramide synthase 5 [Source:ZFIN;Acc:ZDB-GENE-030131-1740]
Coordinates
chr22:5733938-5735710:-
Coord C1 exon
chr22:5735582-5735710
Coord A exon
chr22:5734045-5735581
Coord C2 exon
chr22:5733938-5734044
Length
1537 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAAA
5' ss Score
7.3
3' ss Seq
TGTTTTCCCTCTTCTTACAGGCG
3' ss Score
12.12
Exon sequences
Seq C1 exon
GATTTCCTCATCATGTTCATTCATCATCTGGCCACCATCGGCCTGATCAGTTTCTCATATGTAAATAACATGTTGCGTGTGGGAAGTTTGGTCATGTGTGTTCATGATGCCTCCGACTTCCTGCTGGAG
Seq A exon
GTAAAAAAAACAACAACAACAAAACACTTGCACTTCTGCATATATATATATATATATATATATATATATATATATATATATATATATAATTGATTAAGATCTAAACATTTTTCCTATATATACTGTAACTAACTCTAAATGTAAATAATTATAATGTAATTATAATTAAAAATAATTTTATAAATATAATGTAAATAATTCTAAATAATTTGTTTTATGCTTTCAGTTGTCATTTTTTTTAAATAAAACAAATATCAAAATTTTACAGAAACATATTATTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGTATATATATATGTATGTATATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTATGTATGTATGTATGTATGTATATAGACATATATCATGTATAGACATATATCATGTATATATCTTGGCATATATATATATATGCCAAGGATTTTCCAGTTAAAGATAATTTTTAATATTTAATATCTTTATTTATTCTCCTTTTAGGCTTTGAATAACTGATTCATACATTATTTATTTGTTTTCATTAAATATTTTTCAAAAGCTGAAAAAGATATATACAAATCTATGAAGTATATATATTATAAAAGTAGAGTATCCTTAAAAAATGCATGTATGTTTGATGGATTCAGTTCTACATATTTAATGTTTAATTTTTCATAGCCTATAATTTTGCAATGTATAATTTTTATGTTCATTTAATTTATTAATATCTAAAGATAATCATTATATATGTCAATAATTGATTTTTTTTGTAAATAAATACAAAACATTTTTTTGCAATATTTCCAGTTACCTCCACAATTTTGCATATTATTTAGCTAATATCAGTGTTTAAAAACACTAGAATAGAAATAGCACTGTTTGCAAATGTTTCTTTTCTAAATTGTAAGTTATGTTAGCAACTTTGGATAGAAATTTAGCTTTCAAACACATTGGTTTGAAAAAGACACTGTTTTCTACATTTTTCTTCAATATTGCAAGTTACTTTGCATATTTGTATGGAAATTTGACTGTTGTACTTTTTACGTTTCGAAAATGTTAGGATGAAAATAGTGCTTTATTTTCAAGTGTTTTCTGTAGTATTCCATGTTAATGATTGATTTTTTTTTTTTTTTTGGACAAAAAAGCTTTTAAATAGCACTAATCTACAAATCCTTCAAGAATATATTAGAAAAATATAGTATCATCCTCTCAAAATCACCAAAAATTTATCGTCTTATTCGAATTGTTATTCTAATAATTTATATTAAGAATCTGTTTGAGAAGTGCTGAAGTATAATTCTATCCTATTGACTTTTCACTGTTATTTTCTTTGATATTTATGTTTTCCCTCTTCTTACAG
Seq C2 exon
GCGGCAAAGTTAGCAAACTATGCCAAATACCAGCGTGTGTGTGACGTGGTGTTTGTGATATTCGGGATAATCTTCTTCGGGACGAGATTGATCATTTTCCCCTTCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000002365:ENSDART00000011076:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0379811=TRAM_LAG1_CLN8=FE(21.6=100)
A:
NA
C2:
PF0379811=TRAM_LAG1_CLN8=FE(18.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCATCATGTTCATTCATCATCTGGC
R:
CAGAAGGGGAAAATGATCAATCTCG
Band lengths:
230-1767
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]