DreINT0053254 @ danRer10
Intron Retention
Gene
ENSDARG00000068680 | ctrl
Description
chymotrypsin-like [Source:ZFIN;Acc:ZDB-GENE-040912-147]
Coordinates
chr15:37921838-37922230:-
Coord C1 exon
chr15:37922050-37922230
Coord A exon
chr15:37921969-37922049
Coord C2 exon
chr15:37921838-37921968
Length
81 bp
Sequences
Splice sites
5' ss Seq
CCTGTAAAT
5' ss Score
-0.64
3' ss Seq
TTCTTCTTTTGTTTTCACAGCAA
3' ss Score
9.29
Exon sequences
Seq C1 exon
GCCATCACTCATCCTTACTACAATAGTCAGAACTTCAACAATGACATTACCCTGCTGAAACTGTCCTCTCCAGCCCAGCTGACGTCTCGCATCTCTCCTGTGTGTCTGGCGGCCTCCTCCACCAGCATCCCATCTGGAACTCGCTGCGTCACCACTGGATGGGGCAAAACTGGAAGCACCT
Seq A exon
GTAAATATAGCTCAATTAATGCCAATTCAATACCTGTCACACAATGACTATGCCTTAGATTTTCTTCTTTTGTTTTCACAG
Seq C2 exon
CAAGCCCTCGTATCCTGCAGCAGACCGCATTGCCTCTTCTGAGTCCAGCTCAGTGTAAGCAATACTGGGGCCAGAACAGAATCACAGACGCCATGATCTGTGCTGGAGCCTCCGGTGTCTCCTCTTGCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000068680:ENSDART00000099425:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.016 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0008921=Trypsin=FE(26.9=100)
A:
NA
C2:
PF0008921=Trypsin=FE(19.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCGCATCTCTCCTGTGTGTCT
R:
GTGATTCTGTTCTGGCCCCAG
Band lengths:
180-261
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]