DreINT0054258 @ danRer10
Intron Retention
Gene
ENSDARG00000042953 | cyp2n13
Description
cytochrome P450, family 2, subfamily N, polypeptide 13 [Source:ZFIN;Acc:ZDB-GENE-041001-158]
Coordinates
chr20:25617897-25618832:-
Coord C1 exon
chr20:25618691-25618832
Coord A exon
chr20:25618085-25618690
Coord C2 exon
chr20:25617897-25618084
Length
606 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAAA
5' ss Score
8.38
3' ss Seq
ATATACTTTGTTATTTGTAGAAA
3' ss Score
7.02
Exon sequences
Seq C1 exon
AAACCCCATGAAAGAGTAAACGGCTTTGAGGAACCAAACCTTGTTGCATGTGTTCTTGACCTGTTTCTGGCCGGAACTGAATCCACATCCAATACATTATGTTGGGGGCTCATATACCTCATAATGTATCCAGACGTACAAG
Seq A exon
GTAAAAATGAATTATGGTCAGTAATGAATATTGAGTCATAAGTTTCTGTGGATGTACAATTTGTGTTTGAGCAAAAAGAGCAACTTTTGTTTTGTTCTGCTGCAAACTAGTGATACAATGTTCTCCAAACTGTAATCTAGAGCATACAATGACAATTGGTCAAAATAACAACAAAAAGTTTCCTTTTGAATGATTCATATTTTAGACAACAGAATACTGTTTTACTTTAAAAGAGTTTAAAGGATAGCTGATCACCTAAACACAAAGATTATGCCATCATTTACTCACTCTTATAGTTCCAAACTTGTTTGATTCCCCTTCCCTTTTCGGTTGAACACAAAATAAAATACTTTGATGAATGCTGAAGGCCTGTAACCATTGGCATCCATAATAAAAAGAAATTATGCAAGTCAATGGTTACAAGTTTCCAACATTCTTAAAAAAATACTTCTTTTCAACAACAACAACAACAATCCTTGTATATAGAGTAGAAAGAAATGTTACCAGGCCTTTAAACAAGTATTAACATTGTACACAAGGACAAAACTAATAAATCAATATATTTGTACATTCAGCTGTCTTTTGAATATACTTTGTTATTTGTAG
Seq C2 exon
AAAAGGTCCAGGAGGAGATCGACAGAGTGATTGGAAACTCAAGAGAGCCAAGTATAGCCGACAAACCCAACATGCCCTACACAGAGGCTGTCATCCATGAGATCATGAGATTTGGAGATGTTATACCATTGAATGGATTGCGAGTGGCTGCAAGAGACACAACCCTTGGAGAATGCTTCATCCCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000042953:ENSDART00000063058:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.079
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(10.3=100)
A:
NA
C2:
PF0006717=p450=FE(13.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACCCCATGAAAGAGTAAACGGC
R:
TTGGGATGAAGCATTCTCCAAGG
Band lengths:
326-932
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]