DreINT0054420 @ danRer10
Intron Retention
Gene
ENSDARG00000004964 | cyp4t8
Description
cytochrome P450, family 4, subfamily T, polypeptide 8 [Source:ZFIN;Acc:ZDB-GENE-031219-3]
Coordinates
chr16:4805131-4807271:-
Coord C1 exon
chr16:4807144-4807271
Coord A exon
chr16:4805256-4807143
Coord C2 exon
chr16:4805131-4805255
Length
1888 bp
Sequences
Splice sites
5' ss Seq
CTTGTACGT
5' ss Score
5.14
3' ss Seq
TGATTGTTGTTGTCTTCTAGGAT
3' ss Score
11.13
Exon sequences
Seq C1 exon
GCGATGGCTTACTGGTGTCCACTGGACAGAAATGGTTCCGGCATAGACGACTGCTCACTCCTGGTTTCCATTATGACGTTTTGAAGCCTTACGTGAAACTTATTTCTGATTCAACTAAAGTCATGCTT
Seq A exon
GTACGTCAAATGAAAATATTATCATTATGCTTACTCACCATTGTTAAAGTGTTTGTTATGTCAGACTCTACAGTGTATGAGCACACAGAACCTCTGGCTTCAGACAACATCTGCCTGATTTTGGCGCAATCAGTTTAACATAGGCTAGTCGTGGTGTTTGGTAAAATGATCATAGGATTGGCTTAAATTGTAAACACTGTGCCTGTGCTATAATTATATAACTGCTCAGTTTGTTTGAGCAACTTAACTAACATTATAAAGTGCCAGCATGCCCTTTTTGGTTTGTTTATTAGAGGTTTATTAGAGGCTAAAACTAGGCTGTGGTTTTGGCTGAAAATATGAAAGATATCTAACAATAATAAACATATAGACTATTGATCAAACAGACAGGAATGAATGACTACAAATGTGACATCTCTAGAACTTATACATCTAATCACCAGGATTTTACACTCATCTGTGTCTTTTTTAAAAACTTATATAATAATGAACGGAGTAACACTAGTAGCAAAAACTGTTCAAAAGACAAAACGAATAAATAAAAAATCTGAAGACTATTATCATGCTAATTCAAAGCGCGAAAGATGTCAGATGTGCTAATGCTCATTCACAAAAACTACACTCACCGGCCACTTTATTAGGTACATCTTACCAGCACCGGGTAGGACCCACTTTTGCCTTCAGAACGGCCTTAATTCTTAGATTCAACAAGGTACTGGAAATATTCCTCAGACATATTTGTCCATATTGACATGATTGCATGGTTGGTGGAGATTTGTCGGCTGTACATCAATGATGTGAATCTCCCATTCCACCACATCCCAAAGGTGATGACTGTGGATCTGGTGACTGTGGAGCCCATTTGAGTTCAGTGAACTCATTGTCATGTTCAAGAAACCAGTCTGAGATGATTTGTGCTTTATGTCATGGTGCATTATCCTGCTGGAAGTAGCCATCAGAAGATGGAGACACTGTGGTCATAAAGGGATGAACATGGTCAGCAGCAACACTCAGGTAGGCTGTGGTGTTGACACGATGCTCAATTGGTACTGATGGCCTCAAAGTGTGCCAAGAAAATATCCTCCATCCAAATGTGGCAGCAGAAATGGAGACTGGTCAGAGCAGGCAACGTTTCTCCAATCTTCTATTGTCCAGTTTTGGTGAGCCTGTGTGAATTGTAGCCTCAGTGTCCTGTTCTTAGCTGACAGGAGCGGCACCCGGTGTGGTCTTCTGCTGCTGTAGACCATCCGCCTCAAGGTTGGACATGTTGTGTGTTCAGAGATGCTCTTCTGCAGACCTCGGTTGTAACGAGTACTTATTTGAGTTACTGTTGCCTTTCTATCCGCTGGAACCAGTCTGGCCATTCTCCTCTGACCCCTGGCATCAACAAGGCATTTGTGCCCACAGAACTGCCGCTCACTGGATATTGTCTCTTTTTTAGACCATTCTCTGTAAACCCAAGAGATGGTTGTGCATAAAAATCCCAGTAGATCAGCAGTTTCTGAAATACTCAGACCAGCCCGTCTGACACCAACAACCATGTTCAAAGTGACTTAAATCCCCTTTCTTCTCCATTCTGAAGCTCAGTTTGAACTGCAGCAGATCGTCTTGATCATCTCTACATGCCTAAATGCATTGAGTTGCTGCCATGTGATTGGCTGATTAGAAATTAGCGTTAACAAGCAGTTGGACAGGTGTACCTAATAAAGTGGCCGGTGAGTGTAGTGTTCCGAAATGTGAGGTCCTAATAATAATACACAGAATATAGAATAAAACATTGAATTATACATTGTTTTAATTTAAACAGAAATGTTATATTGTTAATAAACTTGGGAACATCATCACAGGACCTTTCGATGAATGCATTGTGATTGTTGTTGTCTTCTAG
Seq C2 exon
GATAAATGGGAAGTTCACTCCAGATCAGAAGAGTCCTTTGAGCTGTTTAAGCATGTAAGTCTCATGACGCTGGACAGCATCATGAAATGTGCATTCAGCTGTAACAGCAACTGTCAGACAGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000004964:ENSDART00000131949:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(10.3=100)
A:
NA
C2:
PF0006717=p450=FE(10.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGATGGCTTACTGGTGTCCAC
R:
GTCTGTCTGACAGTTGCTGTT
Band lengths:
250-2138
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]