RnoINT0044641 @ rn6
Intron Retention
Gene
ENSRNOG00000043513 | Cyp4x1
Description
cytochrome P450, family 4, subfamily x, polypeptide 1 [Source:RGD Symbol;Acc:628719]
Coordinates
chr5:133942319-133944129:-
Coord C1 exon
chr5:133944002-133944129
Coord A exon
chr5:133942447-133944001
Coord C2 exon
chr5:133942319-133942446
Length
1555 bp
Sequences
Splice sites
5' ss Seq
CTGGTAAGT
5' ss Score
10.65
3' ss Seq
ATCTTGGCCTCCTCTGACAGGAT
3' ss Score
8.25
Exon sequences
Seq C1 exon
GAAGAGGACTCCTGAATTTAGATGGGCCCAGGTGGTTCCAACACCGCTGCCTCCTGACTCCTGCGTTCCATCAGGACATCCTAAAACCATGTGTGGACATGATGGCCCACTCTGTGAACATGATGCTG
Seq A exon
GTAAGTGAAACACTAGATTAGACGTTTATATAGTGGCTTGAGAAAAAATCAAAGCTGATTTCTAGAAACTAATCAGAATGCAAGTACCCACTGAGTAATGATGTCTATATAAGGCTTTCCTGAATTCCTCAGGTGATTAAAATACAAGAGCTGGTCCTCTGCCTTAGAGTCTAACACTCTGTGGTGGTAAGACTCAGGTAGCAGGCACTGTGGGAGACAGGTAAGGAAGCCATGTGCAGCTAACTGGCTTCAAATATCAGATTTATCACAAATATAAGCTGACCTGCAGAACAAGAAACATCTAGGCAGAGAGATGTGAGCAAAGGTTTAAGGCTAAAATGGACTTGGCTGATGGGCATGAAGGACTGAGCAAAGGGTAGGTGCCTATCATAGGTGGTGAGGGATCTATAGGTGAGTGGCACCTTCCAAAGGGGCAGGGCACTGCCTTCAAGAGGCATCCCTGTTGCATGCCTTTCTGGTTTAGAGATGAGTTTTATCAAAAGCTCTATTGACATAAAGGTTTCAGTTTTCTTAATGTTAAAAGTAGACAACTAATAAATACAGTTTCTTCACATGCATTTTTATACAAAAAGTGTTTGTCATGATAAATACATTTATAACTGGAGGATGGGCACTGTGGGTAGTTTAGCAGTCGGACAACACATCTCAGGCTGTCTGAGTAAGCCTCTCTCTGTGGGGAGAGCCAGCTCCCCACAGCAACTCCCTTTTCAGGCCTCTTTGTGTTCTCAATGTCTGAATCAGAAATATTTTTACTGTTTTTATTTGCAATTTTTTAGTTAAATATAACTATAGCATCTTCTCTTTCTTCTCCTCCCATCAACTCCTTCTGTGTTATCATCCCCTGTTCCCTCTCAAATCCATGATCTTTTTCATCAATAATTATTGCCTATTCTTTAATAATTATTGTATATATAAATATACAAATACGACCATCACTGTTGTATGTATGTGTTTTCAGAGCTGACCACTTGGTATTGAATAACAGTTTAGGGGGCTTGTTCCTGGGAGAGACTTACTTGCCCTGTCCCATCAGTCATTACTTGCCTGTAGCTCCTCACCTAAAACTGGGGTCTCAGGTGTCCCTCCCCTCCCATGTTAGCATGTCTGTTGGTGCTGTCCATCGCTGAATCGAAGATGATTTTGAAAAGTGTTCTGAGTTTATCTGAAGTGTGGCGAGACAGGTGAGAGCTGTGACGGGGTATCTAGCAGGTTGGGGATTCAAAGTAGAAGCAGCCCCAGGAAATGCCACGTGTTGCAGTCTTAGATGGTGTGTGGAACTCTGTACCATTTGTTGCAGAGCTGAGATAGTTGCTGACAGACTATTTGCCAGACTCGAAACACGAGTCATGCTCTTTCTCACTGGCCTAGCGTGTGCTTCCTTTCCTCACGCCCAGAATAAACACATGGGTGATTTACTCCTGGCCTTCAGTTCCTGTCAGGTCAGGGTTTTGTCCCTGAGACAATAAGGGGAAAAGCAGTCAAGTAAAAGCCATTTGATGATGGTGAGGATATTGATCTTGGCCTCCTCTGACAG
Seq C2 exon
GATAAATGGGAGAAGACGTGGACCACGCAGGAAACAACCATCGAGGTTTTTGAACACATTAACTTGATGACCCTGGACATAATAATGAAATGTGCTTTTGGCCAGGAGACCAACTGCCAGATAAACGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000043513:ENSRNOT00000011985:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(19.6=100)
A:
NA
C2:
PF0006717=p450=FE(19.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAGGACTCCTGAATTTAGATGGG
R:
CCGTTTATCTGGCAGTTGGTCT
Band lengths:
254-1809
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]