DreINT0062411 @ danRer10
Intron Retention
Gene
ENSDARG00000056744 | ela2
Description
elastase 2 [Source:ZFIN;Acc:ZDB-GENE-041117-1]
Coordinates
chr8:21347622-21347961:+
Coord C1 exon
chr8:21347622-21347744
Coord A exon
chr8:21347745-21347824
Coord C2 exon
chr8:21347825-21347961
Length
80 bp
Sequences
Splice sites
5' ss Seq
CCGGTTGGT
5' ss Score
5.21
3' ss Seq
AATGCATGTGTGTCTCTCAGCAA
3' ss Score
6.12
Exon sequences
Seq C1 exon
CAGCCGAACTTACAGAGTCTTACTGGGCAAGCACAACCTGCCACTCAGCAGCGAGTCCGGCTCTCAGGCCATCTCTCCTGCCAGGATTATCGTTCATGAGAACTGGGACTCCTACAATATCCG
Seq A exon
GTTGGTCCTCGTTTCATTTTAAAGCAAGAGAACAGAAGTTTAATCTAAGAGCATGTTTTTAATGCATGTGTGTCTCTCAG
Seq C2 exon
CAATGACATCGCCCTGATCAAGTTGTCAACTCCAGTGACTTTCACTGACAAGATTTCACCTGCTTGTCTGCCTGATTCCGGCAGCATCCTTCCTCACAACTCCCCCTGTTACGTCACCGGCTGGGGACGCCTCTGGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000056744:ENSDART00000136765:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0008921=Trypsin=FE(17.7=100)
A:
NA
C2:
PF0008921=Trypsin=FE(19.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTACTGGGCAAGCACAACCTG
R:
CAGGCAGACAAGCAGGTGAAA
Band lengths:
178-258
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]