DreINT0066282 @ danRer10
Intron Retention
Gene
ENSDARG00000100292 | fam166b
Description
family with sequence similarity 166, member B [Source:ZFIN;Acc:ZDB-GENE-070209-41]
Coordinates
chr10:17122606-17123070:-
Coord C1 exon
chr10:17122847-17123070
Coord A exon
chr10:17122761-17122846
Coord C2 exon
chr10:17122606-17122760
Length
86 bp
Sequences
Splice sites
5' ss Seq
CAGGTTATT
5' ss Score
3.49
3' ss Seq
TTTGTTCACTGTGCTTCAAGGTT
3' ss Score
6.74
Exon sequences
Seq C1 exon
GTATGCAGGCTACTGTCCTCAGCTGAAGTATCATGTGGGCCAGACTTATGGTCAGTTGACAGCAAAGCTCCTAACTTCCCCGGAGGTATCCCACTCACAGAGGCTTGTGCTCCAAACCAGTCCTCTCTCATCCACAGAGAAGGAAACAGCATCCCGCAGTCAGATCTGGTGGAGCAGACATGGGGCAAGCAGGAACCTGGAAACTATGATCCCGGGTTACACAG
Seq A exon
GTTATTAGCAACATGGGCTTTGTCATACTTGGTGTCTACTTTGCAGCACTGTTAACTTTCTCCATATTTGTTCACTGTGCTTCAAG
Seq C2 exon
GTTTCGTCCCATTGCGCCAAAACTACATCTGCAAAACGTATGCAGAGACCTGTCGAGATGCTCTGTCAGAGTTCAACCAGGAGCAAGCAAAGAGACTTCACAACGCTTCTGCTGACCTGTCTTTTCCTGTCATCAACAGTGTCCCAGACTTCAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100292:ENSDART00000170083:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.132 A=NA C2=0.019
Domain overlap (PFAM):
C1:
PF106294=DUF2475=PD(89.6=78.9),PF106294=DUF2475=PU(11.4=10.5)
A:
NA
C2:
PF106294=DUF2475=FE(72.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTATCCCACTCACAGAGGCT
R:
TGCAGATGTAGTTTTGGCGCA
Band lengths:
173-259
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]