DreINT0069086 @ danRer10
Intron Retention
Gene
ENSDARG00000012586 | fmnl2a
Description
formin-like 2a [Source:ZFIN;Acc:ZDB-GENE-081105-70]
Coordinates
chr9:4810551-4812329:-
Coord C1 exon
chr9:4812221-4812329
Coord A exon
chr9:4810628-4812220
Coord C2 exon
chr9:4810551-4810627
Length
1593 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAAT
5' ss Score
8.76
3' ss Seq
TCTCTCTGTCTCTCACTCAGTAT
3' ss Score
8.03
Exon sequences
Seq C1 exon
GTCTAATACGTTACCCAGCAGGAGGACATTGAAGAACTCCAGATTAGTATGTAAGAAAGACGACGTTCATGTGTGCATCATGTGTCTACGGGCTATCATGAACTACCAG
Seq A exon
GTAAATCACAAAACCTTCAATCTGTGTTGGTAAGGATACTGTTGATCATCAGTTGGCACTGTTAACCCTTTAGATAGGCCAGTGCTTAGTTTCTGGATTTGAGGTTAACAGCAAATTAAAGTGTGTGTGTATGTGTGTCTGAGTGTGTGAGAGTGACCTTTACGCACTCACCTTGATGTGTCTGAAAAATTCAGATTATTTATCTCAGCTCTCAGAACTACATGGAGTAAACAAAAACAAAGACTGTGCATTTATGCACCATCAAATGTGCGTATAATGGAAGTCAATGGGGCAAAAACAGCCACCAGCAGTAAATTAAGGAGAAAACACATGAAAATCTAACAATGCATCAAAGCCAATGTTGTTACTAATCGTTCACAAGTCCAAGACTTTGATTTGACTTTAACAATTACTTTTTATATTAAAAATACGCCATTTTGTGTGTTTTTTCACCAAATCAGTGACATCATTTATGAATTTGGCACTTAAAAAGTGAAAATCCTGTCATTTTCTAAACAATTTAGTAGTTTTGATCAGGACTGAGGTTGATTTTTACAGATTTATGAAATATATATACGTATTGTAAAAAAATATTGATCAGATGCATTTTTACAGCAGTTTAATTCAGTGGATGTTTTCATCTCTAACATAACAAAAGGGTAGTAATTTGAACAGTGCACAAGGGTTAATGAAAATACTGATTATATAATACAATTATGTAACCAAGTTTACTTACCTTAAATGTGAAAATCACAGAATTAATCTAAATACATTAGTTTAAAGCAGCGAAACTAAATATTTGGCCTATGTCTGGTGTAGAAACAGCTTCTCAGTTTTACAAATCACCAAAACATTTACTTTTTACAGTTTATCAAGCCCCAAAACATTAACAAAAGCCTGTTGACACACATACAGTGCTCAGCATATATAAGTACACGCCTCACAAATCTCTCTTTTTTAAAGTTTGTATTTTTAATAGGAAGCTCTACAATATTATATTTATGCATATACATTAGATAAGTCAGTACTGAAGCCAAATCTGGCGCTTATCTAACAAAATAACATATGATAACGCTCCAAAAAACTAGTATAGCTAAATTCATATGTTATAGAAAAATATTAAATATTAATAAAAAAAGAAGTAAAATCAAGAGAAGCTAAAAAAAATAAAAAAAAATGTGGTTGAAATTTTGTAGGTTGTCATTTTTTGCGATATTTTGCTTGAATTTAATTGTATTATCTTTCAATTTCTCAATATTTTTGGTGAGTAAAATATGATTTTAATAAATAAATCTGTTTAATAAATCTGTTTTGTTTAAATGCACCAAAAACCCTATAGTCACTGAGAAATGGAAACAATATTCATTTTCAAAATGAGCTGTCCTCAATTATGCTGAGCACTGTATCCAGAGCACAGACAGTATGATCCAGCTCTGCAACAGGTTTCAATCTGGGCCTCACATATACAGAAGGCACAGAAACATGGTGATTTGTGAAAAGCTGTTCGGTCTTCATCACGTTTTATGTTCAGATGATAAATCTTGAAGATCCTGCTATGTTTGCTAATTAGTCCTCTCTCTGTCTCTCACTCAG
Seq C2 exon
TATGGCTTCAACATGGTCATGTCTCACCCTCATGCTGTGAATGAAATTGCACTAAGTCTGAACAATAAGAATCCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000012586:ENSDART00000016814:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.108 A=NA C2=0.038
Domain overlap (PFAM):
C1:
PF063718=Drf_GBD=PU(43.1=83.8)
A:
NA
C2:
PF063718=Drf_GBD=FE(34.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AATACGTTACCCAGCAGGAGGA
R:
CTGGGATTCTTATTGTTCAGACTTAG
Band lengths:
182-1775
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]