DreINT0076115 @ danRer10
Intron Retention
Gene
ENSDARG00000063126 | gusb
Description
glucuronidase, beta [Source:ZFIN;Acc:ZDB-GENE-070705-256]
Coordinates
chr5:1808998-1810000:-
Coord C1 exon
chr5:1809824-1810000
Coord A exon
chr5:1809134-1809823
Coord C2 exon
chr5:1808998-1809133
Length
690 bp
Sequences
Splice sites
5' ss Seq
AGCGTGAGT
5' ss Score
8.2
3' ss Seq
TGTTTGTGTGTGTGATTTAGGAT
3' ss Score
10.52
Exon sequences
Seq C1 exon
GCTCCATATGTGGACGTGGTGTGTGTGAACAGTTATTTCTCCTGGTATCATGATCCGGGTCACACTGAGCTCATCCGGCTGCAGCTCAGCACTCAGTTCGACAACTGGCACGGAAAATACCAGAAGCCCATCATCCAGAGCGAGTACGGAGCTGACGCTGTCCCCGGCCTGCACAGC
Seq A exon
GTGAGTCCCAACAACACACATAGCATATCTATATTCACAATCACAAACCAATATAACACTGATACTTCCCCTTAGCCCTACGCCTTTAAGCTAAAGAGAATTGGAGCACCCCAACCCCTTCACGTGAACGCGCAAAATAAACCGAGGGGGAAGGGGAAGGGCAAAGGGGTAGAATTGGGATTGTAGTAAGCTTCTGAACTGAAATCTCTTTAATGAAAATGCAGCACTGTGTCTAACATAACAGCAGCGGACAACAACTGTTCAGTCAACTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAGAGAAAACAACTAAATTAACCTTCTATGTAAACACGGTTGACAGATGTGCGTGGCTATTCATATATAATTTATAAGAGTATAATTGTGTGTGTGTGTGTGTATGTGTTTTTGTGACATATCAGGACATGAATCTGTATAATGACATAGGTATGACAGGTATTACAAGAAGGTGGTGAATTATGAGGACATTCCTGACGTCCTCATTTCTCCAAAAGCTTATAAAGAGTTTCCTGTGAGGGTTGGGTTTACGGGTAGGGTGGGGTTAGGGCAATGCAGTATAAAAACTATAGAAACCTATGGAATGTCCCCACAATTCACAAAAACAAATGTGTGTTTGTGTGTGTGATTTAG
Seq C2 exon
GATCCGCCCATGATGTTCACAGAGGAGTACCAGCGGGCCGTCCTGCAGAACTACCACAGCGTGTTCGACCAGAAGAGGAAGGAGTTTGTGATTGGAGAACTGATCTGGAACTTCGCCGACTTCATGACGGCTCAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000063126:ENSDART00000091932:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0283612=Glyco_hydro_2_C=FE(19.1=100)
A:
NA
C2:
PF0283612=Glyco_hydro_2_C=FE(14.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CATATGTGGACGTGGTGTGTGT
R:
CGTCATGAAGTCGGCGAAGTT
Band lengths:
302-992
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]