DreINT0084816 @ danRer10
Intron Retention
Gene
ENSDARG00000062565 | kcnh4a
Description
potassium voltage-gated channel, subfamily H (eag-related), member 4a [Source:ZFIN;Acc:ZDB-GENE-131125-34]
Coordinates
chr3:17432280-17434407:+
Coord C1 exon
chr3:17432280-17432487
Coord A exon
chr3:17432488-17434212
Coord C2 exon
chr3:17434213-17434407
Length
1725 bp
Sequences
Splice sites
5' ss Seq
TCGGTGAGG
5' ss Score
8.99
3' ss Seq
ACTCTCCCTGTCCTCTGTAGGCT
3' ss Score
10.79
Exon sequences
Seq C1 exon
ACATCACTGGTGCACTTATTAAAGACGGTGCGTTTGCTGCGTCTCCTGCGTCTGCTGCAGAAGCTGGATGGCTATTCTCAGTACAGCGCTGTGGTCCTGACCCTGCTGATGTCCGTGTTTGCCCTCCTGGCACACTGGTTGGCATGTGTCTGGTATGTCATTGGACGCCAAGAGCTTGCCAGCAACGACCCCCTGACCTGGGATATCG
Seq A exon
GTGAGGGACAATGTAGTTTACCTTTTTATTAAGGGTCAGTTCACTTACAAATGAAAATGTGCTGTTCATTTTCTCACCCTGAATTTTACTACTGAAATTCCAAAATCCTAACCTTTATTACAGGAGCTCTGACTGCTTATAGTACTGTATATTGTGCAGAATGTTTTTTTTGTCAGGTTATATTTACTGTACATTCATTAATACTTTTAAGGAAGTATAACTTTTTAATGATAATAATATTAATAATAATAGTAATGAATGTAAAAAAAAAACAATACAATCATCAGAACTTAAATGAACAGTTGACTGGTCACATATGTCCTTAAGTCACAAATACATAAAACAACAGATAACTTGAACAAATTCATATAAAGATCGGTTTACATGGATTTAAGGCATAAGCACAATCCATTTGTCCCAATATTGCAGGTATTTGTCCATTTGTAGTTTTAATGAAACAGTCACCCTCTGCATATTTTGAACATTAGACACAATATCCCAACCTTTTTAGATTTTGTAAGGGGTTCAAGTTGGAGCCATTTTCAAGTTACAGTTTTCTTGCATCATATTTTTTTGTCATGTATTATTTAGTAAATGATATTATTTGCCCCCTGAAGGAGATTGCCACACTCTTTGTCCGGCGAGTGGGAGATCAAAGTTTTTTTCATCCCAGTTAATATTTTGGTTTTAAAGTCCATGTGAAAGTCTAAATGCCAACACATTTAGAAATAAACAGAATATTGTGGAGGATAAAAGCATGTTGTGTTTTTATTTTTTTACAATATGATATGACATCTTTAAAGCATACAGTCGATTTAACCAATATGACCAAAAATAAATAAATAAATAAACACTTAGAAATGTTTTGTGTGATTTTAGTAAAATAACGATGTTGTAAGCATAAACTTCAAACATGTAATCTCAGATACTGCAGTTGTTTGTTTATTATTTAAAGTTAATATAGCAAAATGTAGAAAGCATCACTGCCTTTGTAGTTCTAGTGCTTATTTATTTATTTAATTATTTTAGATATTTAATTTAGATATTATAGATATTTATTAGATATTTACTTACCTAACATTTTTGTTTTGTTTCCTAAATCATAAAGTATTTTCTAGACAAGCAAAAACTAAAAACATTATTTTTTTTCAGAAATAGGAAGTCAAATTCAGATGAGTTTTCCTTAAAACAACAACAATAATAATAATAATAAATAAATAAATAAATAAAAAATAAATAATCTGCCAGTGGGGTAAATACAATAATCTTGTTTTCAGTTTGAATTCAGATTATTTTACCTAAACTTCTAGCAGGTTATTTAACCCATTTTAAAGGAGCATATCATCTTTTTTTTTCTTTTAAATCTCTGATTTTTTGTCAACAAACAAGACAAAAACTCAAACTCTTTTTTACAGTGTGGAAAACAGGACAAAATACTGATTATGCATGCTTATTTATGTTATTGGCAATAGAGTTTTATGTTCAACAAGGCTGGATTTATTGTATAAAAATACAATAACAACACTAATATTAAATTGTGATATTAATAGTGAAATATAATGACAATTTAAAATATCTGCATTCCATTTAAACAAAAACTATTTCACAGCTGTACATATTATTTGTCAGCTTCACTAAAGCCCACTAACTTAAAGTTAATATGAAGTTAAAGTTTCAATCCCCAAACAATTTTTCCTCTATTTCTACTCTCCCTGTCCTCTGTAG
Seq C2 exon
GCTGGCTGCAGGAGCTGGGCAAGCGTCTGGAGACTCCATACACGAATGGTACAGTTGGCGGGCCGTCTCTGCGCAGCGCCTACATCGCTTCGCTCTACTTCACATTGAGCAGTCTGACTAGCGTCGGCTTCGGAAACGTCTGTGCCAACACAGATGCCGAGAAGATCTTCTCCATCTGCACCATGCTCATCGGGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062565:ENSDART00000153763:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0052026=Ion_trans=FE(33.0=100),PF068148=Lung_7-TM_R=FE(69.0=100)
A:
NA
C2:
PF0052026=Ion_trans=FE(31.1=100),PF068148=Lung_7-TM_R=PD(4.0=6.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTAAAGACGGTGCGTTTGCTG
R:
TCTTCTCGGCATCTGTGTTGG
Band lengths:
355-2080
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]