DreINT0095041 @ danRer10
Intron Retention
Gene
ENSDARG00000079199 | megf6a
Description
multiple EGF-like-domains 6a [Source:ZFIN;Acc:ZDB-GENE-090312-12]
Coordinates
chr23:21193041-21195648:-
Coord C1 exon
chr23:21195520-21195648
Coord A exon
chr23:21193631-21195519
Coord C2 exon
chr23:21193041-21193630
Length
1889 bp
Sequences
Splice sites
5' ss Seq
AAGGTACTT
5' ss Score
8.4
3' ss Seq
CTCGTGTTTGTTGATTACAGGGC
3' ss Score
7.09
Exon sequences
Seq C1 exon
GCTGTGGAGTGAACCGATTTGGACCCGACTGCTCACAGAGATGTGAATGTCTGAACGGAGGTCAGTGTGATCCCCGAAACGGCCGATGCACGTGTCTGAACGGCTGGATCGGGCCCAGCTGTCAACAAG
Seq A exon
GTACTTTTACTACTACTGCATCACACTGGGGTTGCCAACCATCCATTTATTGGCTGGGACGGCCCAAATATTGGTCCTAATTGATTTGTCCTCTATGCAGTCTTGGTTAGGTTACTTAGAGTCATTAATTACTAATTACCATATATTAATTATCATAATACTCACCATTGTGAACTTTATGCCAAAGCTGAATGGCCTTCTCTGAATGTAAGAAGGTGCACCCACTGGATGACTCTGGTCTACAAGGCTCTTCTGGCACTGGTGCCTTCATAAGTATGTTCACTTCTTCATAAGTCAAAAAGCCAGTATGCCCAACGTTTAAGTGAATCTTATCATTTGACTGTTCCTCGGGTCGAACTGAAATAGGGAAGCGAGCCTTTAGTCATGCCACCCCCTCATCCTGGAATACTCTTCAGTCGGAACTAAAAATGTCTAAGCCCAATTACATCTTTTCGCTCTCTCCTTTTAAACAAACAACAACAGTCTATTTCTTAATGTATGTATTTTTAACATATTGTAATGTGGTAAAGCTGATCTCAATGGGTTTTTTATTTGATTAAAATAAGAAACGCACGCGATCACACACACATACACACACACACACACACACACACACACACACACACACACACACACACACATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGTATCTCGGTATATTCCATCAGTAAAAATCTCTCTTGTCTAAATCTTGAGTAAACGTCTGTACATATTGTGCTATTGTATGTGGGCTTCATTCAGTATAATCTTGCATATGTTGATTTTATCTTATGTGTAGATTATATGACAAACTCAAACACCGACCATGACAGAGAGATACTCATTCTTCCTGATGTATGAAATACTGGAAGAGAATCTAAAAGAAAATCCTGAATATGGAACCCGTTGTCTTCAGAAAAGTTGAGATTTTATGTGATTTTATCTGTGTATAATGCTTATTAAAACAATAGCGCAGTTGCTTCAGTACAGTAATCAAGTAAGCGTTGTTTTGCTTCTGTTTTATGAGCGCCCCCTGCTGGTAAAGAATAAATAAGCATTTTTTATTTTGTACAGGAATGTTGCAAATATGGTCAGATCGTCTTTAAAATATATTTTAAAATGATACAATTTAAGTGAAATCACTAACTGCCCAAACTAATATGCTGTATAATTTTTTTGTTTGGATCATGATTACAGTGCAGCCATTGCCTCTAGTTTGAAATCACTGCAGATTGTTCAGTCTGAGATAAACAAATATGCTACTTTTTCTATGCTCATATTTATTTACTATATTATTTTTTGATTATTTCTATATTTAAAAAAAGAATGTAAAAAAAGCATTGAAATGTAATTTTGCTTTTAGTGTGTGGATGAATTTGAAAGTAAGTGAATTTGACAAAGTTGGCAACCCTAATCCACACTTTCCCACTTCGCAAAGAATCTCAACGGGCGCCTTGATGTCAAAAAAGATGTCAGAAATGCAAATTAATTTGATGTTACTTTCACATCCATTTGACAGCCACACATTGATGCTCCTTTGACCACCAAAAAGTAAAATAAGTTGTATCCATCTGAAAGCTTTAACTCCAAATTTACACTGGATTTTGTATCCCTGCAATGTATGTTAACTAATTGTTAACTTATATTGACATCCAACATTGGCATCAAGAAAATCTTTTTTGCAGTGATTTTTTTTGACATCAAGGTAGTCATCTGACATCAGAGCGAATAGCATTTTTGATTTTTTTCCCCTTTTTTTTCTTTTACATTATTTGATGTCATTTTAAAATAAACATGCCCACTGGGGTAATTCTCGTGTTTGTTGATTACAG
Seq C2 exon
GGCTCCTGTCTGCTAGCAAAAGTAAAAGGAAGGACTCGTCAGTATAGCTGTGTTGCCTATGCAGTTCAGTAACACAGACTGTAATCTGAACTGCCTTGAATTTGCAAGTAGACCTTTGGCTAACATTTTAGCCCAGTAGTGTTTTTTTTCTTCAGATCTTTAAATACAAAGGTCAACCAATTTTTCAAGTGACTGACATGGACAAGCTCTTGAAGCAGTTTAGATGGATTAACTTCCATTTATGATCATAAATGATCAACTTTTTCATGGAAAATTGGAAATCCAACGTGAAGGTGACTTTTGATATCAAACATCTCCTGGATGAGGACATACGCAACCTTTTTACGATTTTTTTTTTAACTGATTTTAGCAATATTTGTTTTATGAATATAATAATTGTAAATAAATAATGCCATTCCTACAACGTGATCCTAAAAGATCATGTGCAGTTGTTGAAAGATAGTTTGAGTATGTGCACTGAAAACAAAACAAAACTGTGGTTTAGATATCATTTTCATTCCGAAATTAATAGTAAGTTTCTTGAATAATTTCTGCAGAAATGGCATGTAATGCTGAAATAAAAATGGCAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079199:ENSDART00000143206:33
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.267
Domain overlap (PFAM):
C1:
PF079748=EGF_2=WD(100=70.5)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGCCCAGCTGTCAACAAG
R:
GGTTGCGTATGTCCTCATCCA
Band lengths:
358-2247
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]