DreINT0099787 @ danRer10
Intron Retention
Gene
ENSDARG00000009782 | myh11a
Description
myosin, heavy chain 11a, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-050531-1]
Coordinates
chr6:8276569-8278510:-
Coord C1 exon
chr6:8278387-8278510
Coord A exon
chr6:8276778-8278386
Coord C2 exon
chr6:8276569-8276777
Length
1609 bp
Sequences
Splice sites
5' ss Seq
CAGGTACAT
5' ss Score
7.79
3' ss Seq
ACGTTTTCTTCATCCTTCAGGTC
3' ss Score
11.96
Exon sequences
Seq C1 exon
GTCTGCACTGTCTGATGAGAAGAGACGGCTTGAAGCCAAGATTCAGCAGCTGGAGGAGGAACTGGAGGAGGAGCAAGGCAACATGGAGATGCTCAATGACAGGCTGAGAAAGAGCGCACAGCAG
Seq A exon
GTACATGCGTTACAGTACATCAAATAGACATTAATATTAGGGATGGACCCATTTGGAAATATTGACCTGTTGTGAATTGAAAAAGCGACAGGCTAGGAGACTTTTCGGGTATCTTCAAAGCACAATCCTTTAATGAGAACTTACGGTTGCTGTATTCATCATTTAAAAATCTAAACTAGTGCTGCTTTGCACTCAATTCAAATCACATTTTAGGGGGCAGTGATACATAGAGGTTGACACAAATCTAAACAAAGTATGCAAATGAAGTACAGGAGTCAGCCAAGTACACTTAAAAATAATATTTGCTGTTTGTAAGAGCTACTACTTAATTGCTATCTTAACTATCTTAATTGTTTCATTTTCAATCCAGGACCCTGCCTGATGGTCAGGAAATGCATAAAGACCATAGGTTAAATGTCAACTTGGCCTGCTAGTTTTGAATATTATATTGTTAAAAAAATACTCTTTAGATTTGGAGACCAATATATTGGCCGATAAATATAAATATTATAGATAAAAAAATGTTCTGCATGATTGCAAAAACTAAAGGAAAACACATATAGTAGACAGTTCTGAATGTAATTAATTAACTGTTCTTTTCTGTGGCCAACCTTCTTTATGAATATTATGCAAAAATAAAGCAACAAAGTTGCTGAACATTAACCGAAATCAAAACACCATTCATTCATTCGTTTTCCACCAGCTTATATCCTTTCTTCATTAGGGGTCGCCAGAGCAGAATGAACCGCCAACTAATCCAGTGTATGTTTTACACAGCAGATGCCCTTCCAGCTGCAACCGAGTACTGGGGAAATACCAGTAAAATAATAAAAAATTGGCTGGCAGAGAGGACGTGAATTGGTTTAACCAACTGAAATAATATATAATTTATCGTCCTAAAGATATTGTCTAAAAATATCATTAATTGGCCGATATCGATAAAGCCACTGTGCATCCCTAATTAATATGTCAGGTTTTTATACATATGTCTCTGATCTTAGGTCTCAAATGGTAATATTTCTTTATTGGATTTTTTTATAGGCGTTTCTAAAATTAATTAACTTATTTTTGTTTTAAAATGTTTCCCCATTTAGTATTTGCTCTAATTTCGCTATTAAGAAAATTATACAGTATTTGCAGGTTTCAACTCAAAAATAAATGAACGAATGAATTAATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTGATTAAAGAATAAACGAATGAACAAACAAATTAATGTAAAAAAAAATCCTAGATTATGCCTGTTTATTCCTGGATCCCTTTATAAAATAATTACTTTTTTATTTCAGAGATATTGATTTTTAAAGCTTTCAATTAATTTCCCTCGGCACTAGCAAACAATCCAAATGAGCTTTTTTTTTTTTTAAATGGCGCATTTAGACATAAATACAGTGGTTATTTGCAATGGAAATTCCATTGCAGAAAGAGAGCTTTATGTCATTTGTATAAACAAAAAAAAAAAAAGCCATCTCATTTCCTTAAACGTTTTCTTCATCCTTCAG
Seq C2 exon
GTCGACCAGCTCACCAACGAGCTGCAGGCCGAGCGCACCACATCCCAAAAGAATGAGAGCGCCAGACAGCTGATGGAGAGGCAGAATAAAGAGCTGAAGGCTAAACTGCAGGAGATGGAGAACCAAGTCAAATCCAAATTCAAGTCCTCCATCTCAGCTCTCGAAGCCAAAGTGGCACAACTGGAGGAGCAGCTTGAGCAGGAGAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009782:ENSDART00000041142:37
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.914
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(4.8=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(8.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGTCTGATGAGAAGAGACGGC
R:
CTGCTCTCCTGCTCAAGCTG
Band lengths:
326-1935
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]