DreINT0100070 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40756803-40757285:-
Coord C1 exon
chr6:40757174-40757285
Coord A exon
chr6:40756896-40757173
Coord C2 exon
chr6:40756803-40756895
Length
278 bp
Sequences
Splice sites
5' ss Seq
CAGGTATGT
5' ss Score
9.8
3' ss Seq
CAACATTATGACTCAATTAGGGG
3' ss Score
1.94
Exon sequences
Seq C1 exon
TGGAGAATCTGGTGCAGGAAAGACTGTAAACACCAAACGTGTCATCCAGTACTTTGCGACAATCGCTGTGGCTGGCAAGCAGAAGCAAGAGCCTATTCCTGGAAAAATGCAG
Seq A exon
GTATGTAGAGAAGTTGATTGCTGCAGAGCCTGTTGGTGGGGCTTCATATCCAGCTGTCCCTCCCTAAACGTAATGGGTGGGGCTTGAAGGTCCAACCCTTTCCTTAGATATAACTAGATAAAGCTTCACCCATTAGGTCCACAGAGACAGAGCCAGGCTAGGTCAAGGTCCAACCATTACTTGGATTATCCACATATGGGGTATAAATGGGATTTGTTTGTAAATATGATGAGGATTCATTTTTTACTGGTATGCCTGCAACATTATGACTCAATTAG
Seq C2 exon
GGGTCGCTGGAGGATCAAATCATTGCAGCCAACCCCTTGCTGGAAGCCTATGGCAACGCCAAGACTGTGAGGAATGACAACTCTTCCCGCTTT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.053 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(5.4=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(4.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGAGAATCTGGTGCAGGAAA
R:
AAGCGGGAAGAGTTGTCATTCC
Band lengths:
204-482
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]