DreINT0100071 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40756637-40756895:-
Coord C1 exon
chr6:40756803-40756895
Coord A exon
chr6:40756701-40756802
Coord C2 exon
chr6:40756637-40756700
Length
102 bp
Sequences
Splice sites
5' ss Seq
TTTGTGAGT
5' ss Score
5.91
3' ss Seq
CCTGTGCCTGAATCCTTCAGGGT
3' ss Score
7.85
Exon sequences
Seq C1 exon
GGGTCGCTGGAGGATCAAATCATTGCAGCCAACCCCTTGCTGGAAGCCTATGGCAACGCCAAGACTGTGAGGAATGACAACTCTTCCCGCTTT
Seq A exon
GTGAGTACTGAGCCAACAGCCACTGAGACACTATGTGTTTTTATTCTGTAATGGAAACATTTTACACATTTTATTCTGTTATCCTGTGCCTGAATCCTTCAG
Seq C2 exon
GGTAAATTCATCCGAATTCATTTCGGCACCACTGGAAAACTGGCCTCGGCTGATATTGAAACTT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(4.4=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(3.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGTCGCTGGAGGATCAAATC
R:
GTTTCAATATCAGCCGAGGCCA
Band lengths:
155-257
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]