BtaINT0097078 @ bosTau6
Intron Retention
Gene
ENSBTAG00000018204 | MYH1
Description
Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), mRNA. [Source:RefSeq mRNA;Acc:NM_174117]
Coordinates
chr19:30129802-30130083:-
Coord C1 exon
chr19:30129991-30130083
Coord A exon
chr19:30129866-30129990
Coord C2 exon
chr19:30129802-30129865
Length
125 bp
Sequences
Splice sites
5' ss Seq
TTTGTAAGT
5' ss Score
7.4
3' ss Seq
TCATCTTTTTCACTTTCAAGGGT
3' ss Score
8.33
Exon sequences
Seq C1 exon
GGGACTCTGGAGGATCAGATCATCAGTGCCAACCCCCTGCTGGAGGCTTTTGGCAACGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTT
Seq A exon
GTAAGTCTCTGGTCACAGAAGGGTGTTCTTTTAGATGCCAGAGAGGGCTGTCTGTGTTATTATTTCAATCTCTTTTACTGTGTGCTTCCAGTTTTATATATACCATCATCTTTTTCACTTTCAAG
Seq C2 exon
GGTAAATTCATCAGGATCCACTTTGGTACCACAGGGAAACTGGCTTCTGCTGATATTGAAACAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000018204:ENSBTAT00000009327:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.065 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(4.4=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(3.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGACTCTGGAGGATCAGATCA
R:
TGTTTCAATATCAGCAGAAGCCAGT
Band lengths:
156-281
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development