DreINT0103441 @ danRer10
Intron Retention
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23047928-23049108:-
Coord C1 exon
chr9:23049004-23049108
Coord A exon
chr9:23048036-23049003
Coord C2 exon
chr9:23047928-23048035
Length
968 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGT
5' ss Score
11.08
3' ss Seq
GTTATCATGTTTGACTACAGAAA
3' ss Score
5.1
Exon sequences
Seq C1 exon
CAAGCATACACAGATGCTTGGAACAAGGACAAGATAAAGGTCCATATCATGCCTGATAACCCTGAAATCCTTCTTGCAAAGGCTAATGCATACAACATAAGTGAG
Seq A exon
GTAAGTGTGTACATCCAAAACACTTGTTGAATAACTTTATGCAGAATATTCCCAGGTTCTCTGCCTCTTAATAAACTCTGGGTATTGGGAAAAAAGTCCCATTATTTGTCTACTTATAAACATAGTCATTGTTTCTCAAAACAAGACGTAACAGCAGTGTTTCATTTTTTGAACAGAAAAGTATCATAGTTTTTTTTTTATCACTTACAGGTGTACCGATAAATTGCATATCATATTAAGAATATTAGAGTACACAAAACAATACACTTCTTGGGTATTTTATATGGATGCTAGGATTGATATTGGCTGATAATTACATTTTATGACTCTTAGGTTACATTAATAGAGCAGTAAAGATATCACTAGTACAAATCCCCTTTTCACTCATTGAGTGATCTGCTGTCAATAGAGGAGTAATATATTTGAAAGGCTTTGCATGTATTAAGATATTATGTGCATCATCTTATATTTATTCTCTTAGGTAGGGCAAGATCTTCTCTTAAGGTTCATACTTAGAGGAAAATGTGCTTAATGCATTATAAAGCTTGAAGCATTAGAATCAATACTCAGTATTGGCCGATCACCATGACATAGAATTGGCACTTGGTGTTGGTTGCAAAATTCCTGATCGGAGCATCCCTAGTATTTAACCATCTTTGTAGTTACCTCACAGAACTGGGCTACTTTTGCACTGTTGCCACAGGTGTTTTAGTCCCTGGGTTCAACTGAGGAAAAACTCTATGATTTTAACCACGGAGAATCACTAAAACACAATTCAAACCAGTGATGTTTGGCATTTCAAATGTGCCATCTTTGCCAATCATAAACAAACTAACAGCTCAGATAATATCAATTACTATTATCAACGTAACTCTTTATTGACAATTATTGACTTTATCATCGTCTTCTCTTTTATTATGTTTGTTCAGAACTTGTATAACCTGTGCTGTTATCATGTTTGACTACAG
Seq C2 exon
AAAGCCTACAAAGCAGGAATGGAAGCTCTGAAGAAGAAAGGTTATGACCTCAAGGCAGATGCTGTATCGATCATTGCAGCTAGGGCCTCCACAAACATTGCCAGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630:ENSDART00000143888:39
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAGCATACACAGATGCTTGGA
R:
GCAATGTTTGTGGAGGCCCTA
Band lengths:
206-1174
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]