DreINT0111628 @ danRer10
Intron Retention
Gene
ENSDARG00000003449 | pde10a
Description
phosphodiesterase 10A [Source:ZFIN;Acc:ZDB-GENE-040426-1115]
Coordinates
chr13:4477524-4479668:+
Coord C1 exon
chr13:4477524-4477607
Coord A exon
chr13:4477608-4479517
Coord C2 exon
chr13:4479518-4479668
Length
1910 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGA
5' ss Score
10.77
3' ss Seq
GTGTGTGTGTGTAATTAAAGCTT
3' ss Score
3.98
Exon sequences
Seq C1 exon
GTTTCACTTTGACATTGCACCGTTCGAGGAGCTGTGGCCTGCAATATTCGTATACATGGTTCATAATTCATGTGGAAAAACCAG
Seq A exon
GTAAGAGTGTGTCCGCGTCTACAGGTACATATCAAATTATTTCAATATCATGCACATGTTTATTTTTTTGCAATTTAATAAAGAAAGCGTGCTTCCTTATATTCTAAGCTCACTGCACTCTGAATTAAATTTACATTTCTTTTTGAGATACGACATATTTTTACAACAATTTTATTCAATACAATTTTACTACAATATTTTATTATATATATTAATTTTCGGCTTAGTCCCTTTATTCATCAGGGGTCGCCACAGCGGAATGAACCACCAACTTATCCAGCATATGTTTTACACAGCGGATGTCCTTTCAGATGCAACCCAGTACTGGGAAACACTCATACACACTCTTCCACACACATACACGAGGGCCAATTTAGTTTATTAAATTCACCTATAGAGGAATTACCAACCTACGTCACACGCTTGCGAAAAAGAGACCAGTTACAATAGCAAACATGTCATGTTGTGCAGTAGGACGCCAAAAACAAATTCAAAAATCCAAAAATAGAAAACTTAACATTTACCGTACACCACACTGCTATTGCCAACCGCAGATGTCTTTGGCTAAAAGGGAGCTGAATAAAGTGAAGACCTAATTAAAAATGCTGGATTCTGCAGTTCTCATTTCATGTGAGGTAAGTTCACACTATGCTGAATCATACACATTACTCGTTGTTGATTGCAGCAATGATTTTAGCAAAAACAGTTAAATATGATTAATTAATCTGCGGACACTGAATGTTTAGTGTCCGCAGAATGAAACGTAAAAGTGTAAGTTCACATACCTTGCCGTACAGGTGCAGTTCGCTGACAGAATGCAGTTGTTTTGCTTGTTGATGATTACCCAGGCCTTGTACAGCTCCGTCTTCTTTTCCTTGTCTTTGGCTAGGCAAAACCTTGGTTTTTAGCACTACATAGTTTTGAATCTGGTAATCATGGAACATTATTTCTGACACATGACCACACTGAACAAAATAGTTGGCATCCAAAGACTTGTAGGCCTTTAACTTCTCTCTTGTAAAAACACTTGGAGCTTCTATGAGATTGTATATTTGGGGCTGGATGCTTGGCCATTTCGTCTTATCCAATATCCTTTGGGAGGGTGCAATCGCAAATGGACCTGTCAGATGAACGTCGCTCATTTCATGTTAACTTTGCCGAATCACTGTGCTTATTACTGGGTGACGAGCTGCTATAATACGCTAAAAGCATTATGTAAATAATATATATATATATAACATGTAATCAATATAACTTATTTCTAAGACAACAACAAACTCCGTACCGCATCGGATGTCATTGCCTCGCTGTAATAGCGCTCCCAGTGTTTTCTGCAAACTCGCTGCATGCACATCTTGAATATTTACAAATATGTTAATTCCTCTTTAGCATGTGTTTGGATTGTGGGGGAAACCGGAGCCCCCAGAGGAAACCCACACCAACACGGGGAGAACATGCACACTCCACACAGAAATGCCCATGCTGGAATAGTTGGCGGTTCATTCCGCTGTGGCGACCGTTGATAAATAAAGGACTAAAGCAAAGGAAATTAATTAAATTTTCCCCACATGTACTTAATTTATTTGTTTATTCATTCTTTTTTTAAAGAGTAGAATGAAGCAATCTTTTAAGATCAGTCTTTTGGACATGTCCATAGATGAAGAAGCACTTGTTTACAGTACTGCTTTAAGAAGGCAAAGGTGTAAAGTCATTTTTTATTGTGCGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGTGTGTGCGTGTGTGTGTGTAATTAAAG
Seq C2 exon
CTTTGAGTTGGAGAAACTGTGCCGCTTCACTATGTCAGTGCGGAAAAATTACAGAAGAGTTCCATACCACAACTGGAAACATGCTGTGACGGTGGCACACTGCATGTACGCCATTTTGCAAAAGACCACTGGGATTTTTACTGAGCTTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000003449:ENSDART00000144312:15
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0023314=PDEase_I=PU(11.9=54.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTTCACTTTGACATTGCACCGT
R:
AGCTCAGTAAAAATCCCAGTGGT
Band lengths:
231-2141
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]