DreINT0114200 @ danRer10
Intron Retention
Gene
ENSDARG00000060841 | pik3c2a
Description
phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha [Source:ZFIN;Acc:ZDB-GENE-030328-39]
Coordinates
chr18:27081288-27081702:+
Coord C1 exon
chr18:27081288-27081457
Coord A exon
chr18:27081458-27081545
Coord C2 exon
chr18:27081546-27081702
Length
88 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAAT
5' ss Score
6.38
3' ss Seq
ATCGTAATTGTTTCCTATAGGGA
3' ss Score
7.56
Exon sequences
Seq C1 exon
GCGTCTGAGAATTTTATCTACACCTGTGCTGGATGCTGTGTGGCCACTTACATCCTCGGCATCTGCGATCGTCACAACGACAACATCATGCTGCGCTCCACTGGCCACATGTTCCACATAGACTTCGGGAAGTTCCTGGGTCACGCACAAATGATTGGGAGTTTTAAAAG
Seq A exon
GTGAATGAGAAGTTACAGTATTTAAAAATACGGCACATTATGTAGCATCTTTTAGTTTTATTAATTGTATCGTAATTGTTTCCTATAG
Seq C2 exon
GGACCGAGCACCGTTTGTCTTAACCTCTGACATGGCTTATGTCATCAATGGAGGCGAAAGACCCACTAGTCGCTTTCAGCTGTTCGTTGACCTTTGCTCGCAAGCCTACAACCTGATCCGCAAACACTCCAACCTCTTCCTCAACCTGCTATCTCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000060841:ENSDART00000133547:24
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0045422=PI3_PI4_kinase=FE(25.9=100)
A:
NA
C2:
PF0045422=PI3_PI4_kinase=FE(24.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAACGACAACATCATGCTGCG
R:
GCTGAAAGCGACTAGTGGGTC
Band lengths:
176-264
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]