DreINT0132537 @ danRer10
Intron Retention
Gene
ENSDARG00000011672 | sema3b
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B [Source:ZFIN;Acc:ZDB-GENE-081105-78]
Coordinates
chr8:26576276-26576599:-
Coord C1 exon
chr8:26576480-26576599
Coord A exon
chr8:26576370-26576479
Coord C2 exon
chr8:26576276-26576369
Length
110 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGT
5' ss Score
11.08
3' ss Seq
AGTTTGCTTTTGCTCCACAGGAT
3' ss Score
8.85
Exon sequences
Seq C1 exon
ACGGATTGCGTAAACTATGTGAAAGTGCTACACCACTATAATCGAACTCACCTATACGCCTGTGGTACGGGGGCTTTCCACCCCACCTGTGCTTATGTGGAGGTGGGACAGAAAATGGAG
Seq A exon
GTAAGTTTGTGTGTTTGGGGGTACTGTATATGATTGAGCTTGTGTGAAATCCAGTCCTGTTTGGTTTTGCATGGCCAAACACAATGCTAAAGTTTGCTTTTGCTCCACAG
Seq C2 exon
GATCATGTATTTAGAATCGATCCCTCTCTTGTAGAAGATGGAAAAGGGAAGAGTCCATATGACCCACGTCACACATCAGCTTCTGTTCTGATTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000011672:ENSDART00000027301:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.219
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(8.8=100)
A:
NA
C2:
PF0140314=Sema=FE(7.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGGATTGCGTAAACTATGTGA
R:
TCAGAACAGAAGCTGATGTGTGAC
Band lengths:
211-321
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]