DreINT0161356 @ danRer10
Intron Retention
Gene
ENSDARG00000105142 | tcirg1b
Description
T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3b [Source:ZFIN;Acc:ZDB-GENE-040426-2022]
Coordinates
chr14:16490528-16491072:-
Coord C1 exon
chr14:16490915-16491072
Coord A exon
chr14:16490619-16490914
Coord C2 exon
chr14:16490528-16490618
Length
296 bp
Sequences
Splice sites
5' ss Seq
GACGTAAGA
5' ss Score
7.3
3' ss Seq
GACAATCTCTCTGTGTGCAGTGA
3' ss Score
7.61
Exon sequences
Seq C1 exon
ATCTGGCGTATGATGTTTGGGGGCCGATATCTCATTTTGTTGATGGGACTGTTTTCCATCTACACCGGAGCCATTTATAACGAGTGTTTTAGCAAAGGCCTCAGCACCTTCTCTTCAGGATGGCATGTCCGGCCTAATGCTGAATTCTACAACTGGAC
Seq A exon
GTAAGACACATAAACACACACACACACACACACACGTTTGTTTTTGTGAATTGTTGGTACATTCATAGGTTTCCATTTAATTTTCATTAAACCCGATCCTCACCATGGAAATGAAACTCATTCTGTATGATTTTATAAGCCTTTTGTAAAAATGTTGACATGATGCAATGCCATCATAGGTGACCTTATAATTTATCCATGTCATTATACATATTTTCGTCCTGATATGTCAAAAAAACACACTCATATGATCAAAGATATTGCCAGTAAAGTTTTGACAATCTCTCTGTGTGCAG
Seq C2 exon
TGAGGAGACATTCAAAAAGAACATGTATCTCTCTCTGGACCCAAATGTTACTGGTGTTTTCACTGGGCCTTATCCGTTTGGAATTGATCCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000105142:ENSDART00000170758:12
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0149614=V_ATPase_I=FE(6.8=100)
A:
NA
C2:
PF0149614=V_ATPase_I=FE(3.9=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTGGCGTATGATGTTTGGGG
R:
TCAATTCCAAACGGATAAGGCCC
Band lengths:
244-540
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]