DreINT0165399 @ danRer10
Intron Retention
Gene
ENSDARG00000087717 | tmprss5
Description
transmembrane protease, serine 5 [Source:ZFIN;Acc:ZDB-GENE-131121-184]
Coordinates
chr15:18228785-18230868:-
Coord C1 exon
chr15:18230770-18230868
Coord A exon
chr15:18228928-18230769
Coord C2 exon
chr15:18228785-18228927
Length
1842 bp
Sequences
Splice sites
5' ss Seq
ATGGTAATG
5' ss Score
5.46
3' ss Seq
TTGTATTTTTTTTTCACAAGTTC
3' ss Score
5.02
Exon sequences
Seq C1 exon
ACACTATCAGACCTGTATGTCTACCGCAGTATGACCATGATCTACCAGGAGGAACCCAGTGCTGGATCTCTGGTTGGGGATATACACAACCGGATGATG
Seq A exon
GTAATGTTCAGTTGCATAGTTAAATGTGTATCTGTCTGGATTAGGTTTGATTTAGGCCATGACTGAAACACACTAGACACACTATAATGGAGAGAACTCAAATGCAAATGACAGCAGATTCCGGAACCAAAATCAAGTACAAAGCAAAGTCCATCAAGTTATCCCAAAAGGTAACTCCAGATACGGCTGCAAACAGTTGATACAAGCAATTATGAGAGGATGCAGATAGGATTATATTAGTTTATGCTTATACATTAACATACCCTTACAGGATTGGCAATATGCTAATAATTAAAAAAAATACAAGAGCTCATAAAAAATGGCATTTTATCTCTGTACTGTTATTTCACTTGGGCATTAACTGATTCACAAACAAAATGGCAAAATGTATATATTTAAAAAAATCAGAAATAAGATTTTTTTTTTAATTACCATGTTTTAATCTTAATATAATACTGCTGCTACCAGGATTATCAACACAAACTGTGTCGTTTCATTTCACATTTGAACATAAAATGTGCAGGATTATTTCAAAATATTAGACTCAAAGAGCAGAAAGATATAGTCACAGTTCGACCTTAAAGCAAGCTAGCATCCTAAAATCCAATAGTATCTGGATGTAGCCTTTATGATGCAAGCTGTTTTTGAATGTTCTGAACTTTTGTGCTAATCAGGTAACGTTATTGATGATAACAGTCACTTACTGCATTCACCATAAGACAAAGCAGCTCCAACTCACACTAAACACTTGGCTTATGCTAGTTTTGTTGAATAACATCAGCAAACAATGCAAAAGAAATATGACAACGAGATGCTGCGCTGCCAGAAACTTGTATTATTGTCGGCTAATGTTAGTGAAAGAGTCGTTCGGGGGATTCATTCACAAACGAGTCGCTCCGTCAGTATGAGAAGTGAAAGCAGGAGAGAAGCTGTGTTTCAGGACATGATTAGATCAAATTTAACAGGGAGGGTGAATAGTACATTTCTGTACACACAAACACAAGCTTTTTGTCAGGAATGCCCGTGCGGTCACTGATCCATCAATGTAGAAAAGTGATGTAAAATTATAATTTTCATAATTAGAAAAAAAAATTACATACTAACATCCAGGAAAACTCCCGATCATAGATATATGTGTATATGTGTATATCTCTGGCTTTGGATGGCCACAGTCCTCCACTGTACATTGGTCCCGCATTCATTTCAAAGGAGCGCTACCCTGTAGCAAGATGGCGGCGCTATTGACGCATTCCGTCCAATAGACAACAATAGGCCAGGCGACATCTAGTGTATATATCTATGGCACTGCACCAGGTCCCTAAAAGAGGGGGGAAAAGGGTGTAAAAAAAATCTCCCTTATATACTTGGAATCGAATTAACCAAATTGTGTCCAAATACCAGACGTAAACAAGGCCTAAACATGAGCAGAGATGTACCTACCTACAGCAGATCATTTAAACAAATGAGGCAAACAAAAATGCAATAGGCAGAAGTCAGAGAGCAAGCACATGTAAACAAGACTAATAAATCCTACCATTAGGAAAATACTTAATAAATTAATACCTAAAAAGACTGGAAATAATGGTCACAACGTGTTAAACAATGAGTAAATAACAGAGCAACAGACAACTCGCAGGTTAAAGTTAAAAAACGACACGAATTGGCGGGAAACTGAAATGAAACGCAATGAGGCCCTCTAGTGGATAAAACAACTCCATAATAACTTAAGTTTATGAGTTCATTTGAGTTCAATACTATTTAACAATTTATTTGAGTTAAAATCTGTCACGAAACATTTTACTGTTTGTATTTGTAATTGTATTTGTATTTTTTTTTCACAAG
Seq C2 exon
TTCTTATACCAGAAGTTCTCAAAGAGGCACCCGTACCTTTAATAAGCACAAAGAAATGCAATAGCTCTTGCATGTACAATGGAGAGATCACCTCTCGAATGCTCTGCGCAGGATACTCTGAAGGAAAAGTAGACGCATGTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000087717:ENSDART00000156505:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0008921=Trypsin=FE(14.2=100)
A:
NA
C2:
PF0008921=Trypsin=FE(20.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TATCAGACCTGTATGTCTACCGCA
R:
CTGACATGCGTCTACTTTTCCT
Band lengths:
238-2080
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]