GgaINT0030095 @ galGal4
Intron Retention
Gene
ENSGALG00000003014 | CHRNA3
Description
Neuronal acetylcholine receptor subunit alpha-3 [Source:UniProtKB/Swiss-Prot;Acc:P09481]
Coordinates
chr10:3126118-3127878:+
Coord C1 exon
chr10:3126118-3127129
Coord A exon
chr10:3127130-3127749
Coord C2 exon
chr10:3127750-3127878
Length
620 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGA
5' ss Score
10.06
3' ss Seq
ATATTTGATTTATTTTTCAGATT
3' ss Score
9.95
Exon sequences
Seq C1 exon
TGCAGTTGGGGATTTCCAGGTTGATGACAAGACAAAGGCCCTACTGAAGTACACGGGTGATGTGACCTGGATACCCCCAGCTATATTTAAAAGCTCATGTAAAATAGATGTGACCTACTTCCCATTTGACTATCAGAACTGCACCATGAAGTTTGGTTCCTGGTCTTATGATAAAGCCAAAATTGACTTGGTTTTAATTGGCTCTACAATGAACCTGAAAGATTACTGGGAGAGTGGAGAATGGGCCATTATTAAAGCTCCCGGGTATAAACATGACATTAAATATAACTGCTGTGAAGAGATCTACACAGACATCACATACTCTCTTTACATTAGACGCTTACCTTTATTTTACACTATCAACATGATTATTCCATGTCTGCTGATCTCTTTTCTGACTGTATTAGTTTTCTATTTGCCCTCAGACTGTGGTGAAAAGGTGACTCTCTGTATATCAGTCCTTCTGTCTTTAACGGTGTTTCTTCTTGTTATCACTGAAACCATACCTTCTACTTCCCTGGTGATCCCACTTATTGGGGAATACCTCCTTTTCACTATGATATTTGTAACTCTGTCCATTGTCATTACGGTATTTGTACTTAACGTGCACTACAGAACACCCAAGACACACACCATGCCTGTGTGGGTGAGAACTATCTTCTTGAACTTACTTCCCAGGATCATGTTTATGACAAGGCCGACCAGTGATGAAGAGAATAATCAGAAGCCAAAGCCATTTTACACTTCTGAGTTTTCAAACTTAAATTGCTTCAACAGCTCTGAAATCAAATGCTGCAAAGATGGCTTTGTATGTCAAGACATGGCATGCAGCTGCTGCCAGTACCAGCGAATGAAGTTTTCCGATTTCAGTGGCAATCTCACAAGAAGTTCCAGCTCTGAATCTGTAGATCCTCTGTTTTCATTTTCAGTTCTGTCACCAGAAATGAGAGATGCTATTGAAAGTGTTAAATACATTGCAGAAAATATGAAAATGCAGAACGAAGCAAAAGAG
Seq A exon
GTAAGAATATATTTTACTATTACTCAAACTGCATTTATGATCAGTTTGCTTTGCAGAAAAAAATCAAACAAAAAGACAACACAGACCATTGTTCGGTATAAGTAAATTACGTAAGCTGTATTACAAATGCATGCAACTTTGAATGTAGATAATTTTGGGGAGCAGCTCTCTTGCACAACATTCTGTCTATTGAGAACAAACCTTGCATGAGAATGTAATAAAATTTCAGCATTACCTATGAAGTGTATACCAGATGTATAGATAACTGTGTACCAATTCATCTGCAACATACTGTTTAAAGACCAGTAGTGTATTTCAGTAAGCTTTTCTGGCTTACCTAAGAACTTAAAGCTTTTTACTTTCACTATTGACATACAGAAGTAGTATCCCATGAAGTCTGATGAACAAAATTGGTGAACAACTTTTTGTATGTGAAGCAGCTTTTGGTTTAGCACAAATAGCATATTCAAACAAAGCTGCTTCAAGATTAATTATTTCATCAGTGAAAACATAGGAAAGCTGAAAAAAATTCTTTGCACACTATGTGAAATATTCCTAATTGCTAATGCTAATTATTAATCTAATAAACATACCTACTTAATATTTGATTTATTTTTCAG
Seq C2 exon
ATTCAGGATGACTGGAAATATGTTGCCATGGTCATTGATCGTATTTTTCTATGGGTTTTCATCCTGGTATGTATTCTGGGAACAGCAGGATTGTTCTTACAGCCTCTGATGACTGGAGATGACATGTAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000003014:ENSGALT00000004759:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.020 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=PD(55.6=34.0),PF0293211=Neur_chan_memb=PU(86.7=63.6)
A:
NA
C2:
PF0293211=Neur_chan_memb=PD(12.5=72.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAAATGCTGCAAAGATGGCT
R:
TCTCCAGTCATCAGAGGCTGT
Band lengths:
346-966
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]