GgaINT0068011 @ galGal4
Intron Retention
Gene
ENSGALG00000008088 | ACSL4
Description
acyl-CoA synthetase long-chain family member 4 [Source:HGNC Symbol;Acc:HGNC:3571]
Coordinates
chr4:13743552-13745121:+
Coord C1 exon
chr4:13743552-13743729
Coord A exon
chr4:13743730-13745011
Coord C2 exon
chr4:13745012-13745121
Length
1282 bp
Sequences
Splice sites
5' ss Seq
CTCGTGAGT
5' ss Score
8.41
3' ss Seq
TTCTCTCTTGAATGTAACAGTTG
3' ss Score
5.02
Exon sequences
Seq C1 exon
TTAATCTTAGGAACCTACAGATGGCTGTCCTATGAAGAAGTAAATCAGAAAGTGAATCGCTTGGGGAGCGGACTGATAGCCCTGGGACTAACCCCAAAGAGCACCGTTGTTATCTTTTGCGAGACCCGAGCAGAATGGATGATCGCAGCCCTGGCCTGCTTCAAGTACAACTTTCCTC
Seq A exon
GTGAGTGCTGAACCTGTGTGCTTTCAATATATTTTAGAATCGATTTCCAGTACTGCTTTGGCTGTTAAGAATGTGTCAGTCCTGATGCTGATAGTATAAGAATTAAACTTATTCTGTGTATCGCCTGAGTTTGGAAGTTTCCAGAATATTCTGTTCATAGTAGAGATCACTACAGTTCACTTGCTCATACTTATGAGCCCAACCTTTGGGACTGCAGCAACATTTTAGATCTTAGCATGACAGATAGGCAGAGTTACTGTTCAACTTTTTCACATACTGTGAGGACTCCGTAGTTGCATTTCCCCCGCAGACTGCGGGATGGTTGTAGCATCTTCTGTCTATATCTGACTTCCATATCTGAAACGTGAAATGGTTACTGGAAGAATGATAACCAAAATGAGATGTTAGGCCGAGGGGGTGGGTATTTTGGTAATGTTGCTGAACTGCTCAAATTCATTACCATTATGTATGTGCTTTTCTTTTTTATTTTATTTAAGCAGTCGGTAATTTAATCTGACTTGTTACATACAGCCTTCTCAGTGAAGTAAGGAAGGTTACTAGAGAGTCATGTAACATTCCTTTTTTTTTACCCTTTCTACAAGATCACAACTTCACAGGAATATTAAATTCTTTAATAGTCTCTGGAGGTTCAAATTTGAGGTGCCAGTACGATACTTAGGGATTTTCCTCAACAGAAAAGGTGGACTCCTCTGTGCCCGTGAGGCACTCAGTAGCATTACTGTCACCAGTCGGGATTTAAAAATAAACTCTCACCACAAAAACCAGCCTGATTTCTTAGTCCGATCTCTGCCCTGCCCCCATCGGTAATTTCATGGGAACAAGAAAGCAGCTGGCACAGAGGCTGAGAGCTGCCACTCAGCTGGAAGTGCACAGCAGGAACGGTGGCTGGTGCCCTGCAGTGTGAGCGTCAGCTCTCCTGCAACATTCTGGTTGCAGAGTGCTCAGAGGAAGAACACATTCTCAGCTGCTTACTGTGCAACTGCTTAGTGTGTTTTCATTGAGTTTTAGTGGATCTTTTTGTTTTGATGATTTCTCTTCTTCCACATAACTGTCCTCATTCCTGTGATCTCTTGTGGAATACAATTCTGATTTGGGAAGATTACAAGCTGCTTTGTTTCCTAATTAAAAAAAAATAATCTCATTGAGCTCACCCCCCCCCCCCCCCGAAGTGCATTGCAGTGCACTAAAGCAAGAGCTTTTGCATTTGTAGTTGCCTTAACTTCTGCATTCATTAACATTCATTCTCTCTTGAATGTAACAG
Seq C2 exon
TTGTTACCTTGTATGCTACACTGGGTGAAGAGGCAGTAACCTATGGTCTCAATGAATGTGGAGCGTCGTATCTGATCACCAGTGCAGAGCTTCTTGAGAGCAAACTTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000008088:ENSGALT00000013126:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(12.4=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(7.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AATCTTAGGAACCTACAGATGGCT
R:
CTTAAGTTTGCTCTCAAGAAGCTCTG
Band lengths:
286-1568
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]